西藏拉鲁湿地水体酵母菌多样性及其与理化因子相关性

Spatial dynamics of yeast community and its relationship with environmental factors in Lhalu Wetland, Tibet

摘要:

[目的]开展拉鲁湿地水体酵母菌多样性研究，探究理化因子与酵母菌群落结构的相互关系。[方法]采用原位培养法从拉鲁湿地11个水样中分离酵母菌，应用26S rRNA D1/D2区域序列分析，并结合经典分类法对获得的菌株进行分类鉴定，运用SPSS和CANOCO软件分析酵母菌多样性及其与环境因子相关性。[结果]从拉鲁湿地水体中分离得到169株酵母菌，鉴定分属为15个属31个种。优势种为Ustilentyloma graminis和Filobasidium magnum，优势属为Naganishia、Ustilentyloma、Filobasidium和Cystofibasidium。统计分析结果表明，化学需氧量（COD）是影响拉鲁湿地水体酵母菌数量的显著因素，另外，此理化因子是影响Ustilentyloma分布的重要环境条件。[结论]西藏拉鲁湿地酵母菌资源比较丰富，且存在明显的空间异质性。

关键词:青藏高原;拉鲁湿地;酵母菌;多样性;理化因子

Abstract:

[Objective] We studied the yeast diversity in Lhalu Wetland and analyzed the influence of environmental factors on yeast community. [Methods] Yeasts were isolated by in-situ cultivation and analyzed by the D1/D2 domain of large subunit (26S) ribosomal RNA and morphological characterization. [Results] In total 169 yeast isolates were identified to 15 genera and 31 species. Ustilentyloma graminis and Filobasidium magnumgenus were the dominating species in the wetland. Moreover, Naganishia, Ustilentyloma, Filobasidium and Cystofibasidium were the dominating genera. COD was significantly positive related to yeast abundance, and it had significant influence on Ustilentyloma. [Conclusion] Yeasts were abundant and diverse among various sites in Lhalu Wetland.

Key words:Qinghai-Tibet plateau;Lhalu Wetland;yeast;diversity;environmental factors