不同配方益生菌在高脂饮食诱导小鼠肥胖发生中的差异化作用

Effects of different probiotics on mice obesity induced by high-fat diet

摘要:

[目的]基于肠道微生物与宿主代谢的相互关系，研究不同配方的益生菌对小鼠肥胖的影响。[方法]50只C57BL/6J雄性小鼠随机平均分成10组，分别给予正常饲料、高脂饲料以及高脂饲料加8种不同配方的益生菌产品（50亿CFU/只），所有动物连续喂养9周，每周测量小鼠体重1次。最后一周测定空腹血糖、葡萄糖耐量试验（glucose tolerance test，GTT）、血脂相关指标，称取内脏重量，并留取小鼠盲肠内容物，提取小鼠肠道菌群总DNA，利用16S rDNA测序检测相关细菌含量。[结果]部分益生菌可引起小鼠体重增速加快，而部分益生菌可减缓小鼠肥胖和降低内脏脂肪重量，同时缓解高血脂症。丹尼斯克品牌益生菌配方组小鼠肠道中厚壁菌/拟杆菌比例（F/B）是正常饮食组的22.8倍，Akkermansia muciniphila（Akkermansia）细菌含量几乎为0；而菌拉丁品牌益生菌配方组小鼠F/B比例与正常饲料饮食组类似，Akkermansia含量为0.5%，为正常饮食对照组小鼠的一半左右。[结论]益生菌可影响小鼠体重和代谢，但不同配方的益生菌效果截然相反。特定的益生菌配方对肥胖和高血脂的改善可能是由于其选用的菌株本身的特性以及菌株之间的相互配比能够降低小鼠肠道中F/B比例以及升高Akkermansia的含量所带来的。此研究为进一步开发可改善代谢的益生菌产品提供了参考。

Abstract:

[Objective] To study the effects of different probiotics on mice obesity based on the interaction between microbiota and host metabolism. [Methods] In total 50 C57BL/6J male mice were equally divided into 10 groups and fed with normal feed, high-fat feed and high-fat feed with eight different probiotics compositions (5 billion CFU/mice). All mice were continuously fed for nine weeks and weighted weekly. Fasting blood glucose, GTT, blood fat indexes and organ weight were measured at last week. Microbiota total DNA was extracted from cecum contents and sequenced by 16S rDNA sequencing. [Results] Some probiotic compositions accelerated mice body weight accumulation, whereas some probiotic compositions reduced obesity, decreased visceral fat and relieved hyperlipemia. Compared to the normal feed group, Firmicutes/Bacteroidetes (F/B) ratio was 22.8-fold in accelerated body weight group fed with Danisco probiotic, and none Akkermansia muciniphila (Akkermansia) was detected, while F/B ratio was similar and the Akkermansia content was 0.5% (half of normal feed group) in reduced obesity group fed with Junlading probiotic. [Conclusion] Some probiotics influence mice body weight and metabolism, but different compositions may lead to opposite effects. Specific probiotic composition improves obesity and hyperlipemia possibly by its selected strain characteristics and relative ratio through decreasing F/B ratio and increasing Akkermansia level in gut microbiota. This study contributes to developing metabolism-improving probiotic product in the future.