微生物VBNC状态形成及复苏机制

Formation and resuscitation of the viable but non-culturable state in microorganisms

摘要:

99%以上的微生物因处于活的但非可培养（viable but non-culturable，VBNC）状态而无法分离培养。复苏促进因子（resuscitation-promoting factors，Rpfs）是培养获取VBNC菌的最重要突破。结合课题组近十余年从环境功能视角利用Rpf复苏培养VBNC菌的研究，本文在阐述微生物VBNC状态的形成及复苏进展的基础上，从VBNC菌形成及复苏过程出发，探究"探索因子"与群体感应的内在关系。并总结了课题组利用Rpf所复苏培养的具有潜在环境功能的VBNC菌种。本论文将为揭示微生物VBNC状态的形成及复苏机制提供新的思路，并为认识和重新评价Rpf法复苏培养VBNC菌在污染环境微生物修复中的作用提供理论依据。

Abstract:

It is estimated that the majority (>99%) of microorganisms resist cultivation because they are in a viable but non-culturable (VBNC) state. Resuscitation-promoting factors (Rpfs) are one of the most important breakthroughs in the cultivation of VBNC bacteria. In the past decade, our group focused on resuscitation of VBNC bacteria by Rpf for exploring their potential environmental functions. Incorporated our previous studies in this mini-reiew, we provided the overview of formation and resuscitation of VBNC bacteria, and explored the relationship between "scout cells" and quorum-sensing based on the process of formation and reversion of VBNC cells. Moreover, we also summarized the VBNC bacteria possessed potential enivronmental functions which were previously isolated from various environments with Rpf addition. This review provides not only a new insight into revealing the the mechanisms of formation and resuscitation of VBNC bacteria, but also a theoretical basis for understanding and re-evaluating the role of Rpf method in the application for environmental bioremediation.