植物乳杆菌JLK0142胞外多糖对RAW264.7巨噬细胞和免疫抑制小鼠的免疫调节作用

Immunoregulatory effect of exopolysaccharide from Lactobacillus plantarum JLK0142 on RAW264.7 macrophages and immunosuppressed mice

摘要:

[目的]研究植物乳杆菌JLK0142胞外多糖（EPS）对RAW264.7巨噬细胞和免疫抑制小鼠免疫活性的影响。[方法]从植物乳杆菌JLK0142培养液中分离纯化EPS，采用体外细胞培养，测定EPS对巨噬细胞增殖、吞噬活性和一氧化氮（NO）分泌的影响；采用环磷酰胺构建免疫抑制小鼠模型，灌胃不同剂量的EPS，分别测定小鼠脾脏指数、T淋巴细胞增殖活力及血清中IL-2和TNF-α水平。[结果]植物乳杆菌JLK0142胞外多糖在50-800 μg/mL浓度范围内能促进正常状态RAW264.7巨噬细胞的增殖，显著提高巨噬细胞的吞噬活性及NO的分泌量；与模型组相比，EPS中、高剂量组小鼠脾脏指数和T淋巴细胞增殖活力显著提高；EPS高剂量组小鼠血清中IL-2和TNF-α含量显著提高。[结论]植物乳杆菌JLK0142胞外多糖能有效提高RAW264.7巨噬细胞的免疫活力，并拮抗环磷酰胺对小鼠免疫功能的抑制作用。

Abstract:

[Objective] To study the immunoregulatory effect of exopolysaccharide (EPS) from Lactobacillus plantarum JLK0142 on the RAW264.7 macrophages and immunosuppressed murine.[Methods] EPS were isolated and purified from the culture medium of L. plantarum JLK0142. The effects of EPS on proliferation, phagocytosis and nitric oxide (NO) secretion of RAW264.7 macrophages were determined by in vitro tests. Then the spleen index, proliferation of T lymphocyte and levels of IL-2 and TNF-α in serum of immunosuppressed mice induced by cyclophosphamide were determined by in vivo tests.[Results] EPS from L. plantarum JLK0142 could improve the proliferation activity of RAW264.7 macrophages in normal state at concentrations between 50 and 800 μg/mL, and significantly improve the phagocytic capacity of macrophages and the secretion of NO. Compared to control mice, the spleen index and T lymphocyte proliferation activity of the immunosuppressed murine were significantly increased in middle-does and high-dose EPS groups, while the levels of IL-2 and TNF-α in serum were also significantly increased in high-dose EPS group.[Conclusion] The EPS from L. plantarum JLK0142 can effectively improve the immune activity of RAW264.7 macrophages and antagonize cyclophosphamide induced immune suppression in mice.