真菌Talaromyces stipitatus WH4-2中的抗菌活性成分

Antibacterial compounds from fungus Talaromyces stipitatus WH4-2

摘要:

[目的]对真菌菌株WH4-2进行鉴定并分离鉴定具有抗菌活性的天然产物。[方法]综合菌落形态和ITS全序列分析，鉴定菌株种属。以抗菌活性分析为导向对其中的活性组分进行分离纯化，波谱分析鉴定结构。采用微量稀释法对化合物进行活性复筛，得最小抑菌浓度MIC值。[结果]真菌菌株WH4-2鉴定为Talaromyces stipitatus。从其土豆发酵液中分离鉴定化合物1（5S-arugosin K）、2（5R-arugosin K）、3（5S-arugosin M）、4（5R-arugosin M）和5（Chrysophanol），其中1-4为含异戊烯基的dibenzo[b，e]oxepinone类化合物，5为蒽醌类化合物。1-4对Staphylococcus aureus ATCC43300、ATCC33591，Enterococcus faecium ATCC35667和Bacillus subtilis ATCC6633具有抗菌活性。[结论]含异戊烯基的Dibenzo[b，e]oxepinone类化合物是真菌T.stipitatus中抗菌活性成分。

Abstract:

[Objective] We isolated and identified the natural products with antibacterial activity from fungal strain WH4-2.[Methods] We identified strain WH4-2 by colonial morphological traits and ITS sequence analysis, used activity-oriented method to isolate secondary metabolites, elucidated structures by spectrographic analysis, adopted micro-dilution method to assay antibacterial activity. [Results] We identified strain WH4-2 as Talaromyces stipitatus. Activity-oriented separation resulted in the isolation of compound 1 (5S-arugosin K), 2 (5R-arugosin K), 3 (5S-arugosin M), 4 (5R-arugosin M) and 5 (Chrysophanol), Compounds 1 through 4 were elucidated as isopentenyl dibenzo[b,e] oxepinone, compound 5 was anthraquinone. Compounds 1 through 4 showed potent antibacterial activities against S. aureus ATCC43300, ATCC33591, E. faecium ATCC35667 and B. subtilis ATCC6633.[Conclusion] The antibacterial compounds isolated from fungus T. stipitatus were isopentenyl dibenzo[b,e] oxepinone enantiomers.