137Cs辐照对橘小实蝇雄虫肠道菌群多样性的影响

Effects of 137Cs radiation on the diversity of bacterial communities in male Bactrocera dorsalis (Hendel)

摘要:

[目的]为研究¹³⁷Cs辐照前后橘小实蝇雄虫肠道菌群多样性和丰度的变化及其肠道菌群在不同分类阶元上的显著差异物种。[方法]本研究利用Illumina HiSeq高通量测序技术对辐照组和对照组共6个样品进行测序分析。[结果]变形菌门和厚壁菌门分别以60.38%、24.33%的比例作为橘小实蝇肠道中的优势菌门和次优势菌门，肠杆菌科和肠球菌科分别以60.38%和15.69%作为优势菌科和次优势菌科。米勒氏菌属（Moellerella）、摩根氏菌属（Morganella）、Cosenzaea属、链球菌科未知属和酸热菌属（Acidothermus）是组间显著差异的菌属。[结论]本研究发现橘小实蝇在辐照后的肠道菌群多样性和丰度均有显著性降低，并在目至属等3个分类阶元上找到辐照后具有显著差异的物种，为后期利用差异菌群来改善辐照对橘小实蝇的消极影响及修复辐照损伤提供理论基础。

关键词:橘小实蝇;肠道菌群;辐照;Illumina HiSeq高通量测序

Abstract:

[Objective] The aim of this research was to analyze the change of diversity and species abundance of bacterial communities in male Bactrocera dorsalis (Hendel) after ¹³⁷Cs radiation treatment.[Methods] We used Illumina HiSeq high-throughput sequencing technology to sequence 6 intestinal samples of Bactrocera dorsalis.[Results] Proteobacteria (60.38%) and Firmicutes (24.33%) were the dominant phylum and subdominant phylum in the intestinal microflora of Bactrocera dorsalis respectively, and Enterobacteriaceae (60.38%) and Enterococcaceae (15.69%) were the dominant family and subdominant family. Moellerella, Morganella, Cosenzaea, unidentified-Streptococcaceae and Acidothermus have significant difference after irradiation.[Conclusion] Irradiation treatment significantly reduced the diversity and abundance of intestinal microflora in Bactrocera dorsalis. And we found significant differential species in different taxonomic level. This result provides a theoretical basis to improve the irradiated-induced damage to Bactrocera dorsalis.

Key words:Bactrocera dorsalis;intestinal microflora;irradiation;Illumina HiSeq