一株深海热液口嗜热芽孢杆菌SY27F代谢产物活性的研究

Evaluating bioactivity of metabolites of a hydrothermal Bacillus sp. SY27F from the deep-sea hydrothermal vent

摘要:

[目的]对一株来源于深海热液口嗜热芽孢杆菌的次生代谢产物进行抑菌活性和抗肿瘤活性的初步研究。[方法]采用纸片法和微量肉汤稀释法检测嗜热芽孢杆菌SY27F次生代谢产物的抑菌活性，采用CCK-8法测定其次生代谢产物的抗肿瘤活性。[结果]抑菌实验表明，嗜热芽孢杆菌代谢产物对大肠杆菌、金黄色葡萄球菌均有抑菌作用，其最低抑菌浓度分别为1.56 mg/mL和3.13 mg/mL；细胞实验表明，其代谢产物对肿瘤细胞A549、HepG2、HeLa、MCF-7均有一定的抑制作用，其半致死浓度分别为0.390、0.451、0.704、1.105 mg/mL；与人肝肿瘤细胞（HepG2）相比，其对人正常肝细胞（L02）表现出良好的生物相容性。[结论]嗜热芽孢杆菌SY27F次生代谢产物具有一定的抑菌和抗肿瘤活性，可为寻找新型抑菌抗肿瘤活性物质提供优质资源。

Abstract:

[Objective] Extracting the metabolites of hydrothermal Bacillus sp. SY27F from deep-sea hydrothermal vents, the antibacterial and antitumor activity of metabolites were assayed.[Methods] The antibacterial activity of the secondary metabolites was determined by the method of paper disk and microbroth dilution method, and the anti-tumor activity of the secondary metabolites was evaluated by CCK-8 method.[Results] The bacteriostatic experiments showed that the metabolites of thermophilic Bacillus sp. SY27F have antibacterial effect on Escherichia coli and Staphylococcus aureus, with MICs of 1.56 mg/mL and 3.13 mg/mL, respectively. Cell cytotoxicity assay showed that the metabolites have the effect of inhibiting cell growth in tumor cells (A549, HepG2, HeLa and MCF-7), with IC₅₀ values of 0.390 mg/mL, 0.451 mg/mL, 0.704 mg/mL and 1.105 mg/mL, respectively. Compared with HepG2, the normal liver cells (L02) showed good biocompatibility.[Conclusion] The metabolites of thermophilic Bacillus sp. SY27F have antibacterial and antitumor activity, and can provide resources for the search of novel compound of antibacterial and antitumor activity.