黄病毒NS2B-NS3pro蛋白酶的结构研究进展

Research progress on structure of Flavivirus NS2B-NS3 protease

摘要:

黄病毒能引起严重的人类疾病，但是并无特定药物来治疗病毒感染。黄病毒非结构蛋白NS3的N端区域及其辅因子NS2B构成蛋白酶，该酶切割病毒的多聚蛋白形成成熟的结构蛋白和非结构蛋白来帮助病毒完成增殖过程。NS2B-NS3pro蛋白酶在黄病毒生命周期中起关键的作用，使之成为抗病毒药物研发的重要靶标。本文综述了黄病毒属中寨卡病毒、登革热病毒、西尼罗病毒的NS2B-NS3pro蛋白酶结构的研究进展，并介绍了相关抑制剂与蛋白酶形成的复合物结构，以期为研发抗黄病毒药物提供必要的参考。

Abstract:

Flavivirus infection causes severe human disease, but no specific drugs are available to treat flavivirus infection. The N terminal region of non-structural protein 3 (NS3) of flavivirus and its cofactor NS2B constitute protease, and the polyprotein produced by virus is cleaved by protease into structural and non-structural proteins that are essential for the replication of the virus. NS2B-NS3pro represents an attractive drug target due to its critical role in the life cycle of flavivirus. Here we review the structural advances of NS2B-NS3pro of Zika Virus, Dengue Virus and West Nile Virus as well as the complex structures of some protease with inhibitors, these will provide necessary references for the future antiviral drug design.