艾纳香内生真菌Diaporthe sp.次生代谢产物

Secondary metabolites of an endophytic fungus Diaporthe sp. derived from Blumea balsamifera (L.) DC.

摘要:

[目的]从艾纳香内生菌J1中获得活性次生代谢产物。[方法]对菌株J1进行ITS序列分子鉴定，综合运用多种色谱技术对其发酵产物进行分离纯化，结合波谱学技术对其进行结构表征。[结果]经构建系统进化树，鉴定菌株J1为Diaporthesp.，从该菌株大米培养基中分离得到7个单体化合物，经鉴定分别为Dicerandrol A（1）、Dicerandrol B（2）、4，6-dihydroxy-1H-isoindole1，3（2H）-dione（3）、Cytochalasin H（4）、Cytochalasin J（5）、4，6-dihydroxy-2，3-dihydro-1H-isoindol-1-one（6）、Cerebroside C（7）。所有化合物均为首次从该菌中分得，化合物1对枯草芽孢杆菌Bacillus subtilis KCTC 1021具有非常强的抑制活性，MIC值为0.125 μg/mL。[结论]Diaporthe sp.富含抑菌活性化合物，具有开发成微生物源农药潜力。

Abstract:

[Objective] To obtain effective secondary metabolites from endophyte J1 originated from Blumea balsamifera (L.) DC. [Methods] The endophytic fungus J1 was identified based on ITS gene phylogenies. Various chromatographic methods were used to isolate and purify compounds from the fermentation extract, and their structures were elucidated by NMR spectroscopy and MS analyses. [Results] J1 was determined as Diaporthe sp. by analyses of phylogenetic tree. Seven compounds were afforded from the rice culture of this fungus, and they were identified to be Dicerandrol A (1), Dicerandrol B (2), 4,6-dihydroxy-1H-isoindole1,3(2H)-dione (3), Cytochalasin H (4), Cytochalasin J (5), 4,6-dihydroxy-2,3-dihydro-1H-isoindol-1-one (6), Cerebroside C (7), individually. All of the seven metabolites were isolated from this fungus for the first time, and compound 1 displays potent inhibitory activity towards Bacillus subtilis KCTC 1021, with an MIC value of 0.125 μg/mL. [Conclusion] This study demonstrated that Diaporthe sp. contains many antimicrobial compounds and may serve as a potential microbial-origin antibacterial agent.