植物根际促生菌Bacillus mycoides Gnyt1铁载体分泌相关功能基因的挖掘

Exploration of functional genes related to iron carrier secretion in the plant growth-promoting rhizobacteria Bacillus mycoides Gnyt1

摘要:

[目的]为挖掘优良促生菌（PGPR）菌株，分析和定位功能基因，发现其科学价值和工业化开发，为农业生产服务。[方法]以Bacillus mycoides Gnyt1菌株为材料，采用二代和三代测序技术相结合的研究体系，对菌株进行全基因组测序研究，分析菌株核基因组可能存在的功能基因和菌株分泌铁载体相关的基因。[结果]本研究基因组序列拼接后总长度为5597907 bp，GC百分含量为35.57%，该菌株中与铁载体分泌相关的基因共9条，其中2条基因主要存在于Porphyrin metabolism途径，与细胞内铁的运输代谢有关。[结论]通过全基因组测序，最终确定了与铁载体分泌相关的功能基因为GYT1和GYT2，为下一步基因功能验证奠定了基础。

Abstract:

[Objective] For the excavation of plant growth promoting rhizobacteria (PGPR) strains, the functional genes were analyzed and mapped in order to find out their scientific value and industrialization development and serve agricultural production. [Methods] Bacillus mycoides Gnyt1 strain was used as the material, and the second and third generation sequencing technology were combined to carry out the whole genome sequencing of the strain. The possible functional genes of the nuclear genome of the strain were analyzed, and genes secreting iron carrier-related strain were analyzed. [Results] The total length of the genomic sequence after splicing was 5597907 bp. The percentage of GC was 35.57%. There were 9 genes involved in the secretion of siderophore in this strain, of which 2 genes mainly existed in the Porphyrin metabolism pathway and were associated with intracellular iron transport metabolism related. [Conclusion] Through whole genome sequencing, the functional genes related to the secretion of siderophores were identified as GYT1 and GYT2, which makes it possible for verifying the gene functions.