饲料类型对茶尺蠖幼虫肠道细菌多样性及幼虫存活率的影响

Effect of feed on gut bacterial diversity and larval survival of Ectropis obliqua (Lepidoptera: Geometridae)

摘要:

[目的]茶尺蠖是茶园中的重要害虫。研究茶尺蠖寄主食物-肠道菌群-茶尺蠖生长发育三者之间的关系对于茶尺蠖的防治具有重要的理论指导价值。[方法]分析不添加茶叶因子的纯人工饲料和茶树鲜叶对茶尺蠖幼虫的存活影响；用高通量测序技术分析不同饲料饲喂的茶尺蠖幼虫的肠道菌群异同。[结果]取食人工饲料的幼虫死亡率远远高于取食茶树鲜叶的幼虫；取食人工饲料的幼虫肠道细菌多样性和丰富度高于取食茶树鲜叶的幼虫；茶尺蠖幼虫肠道中存在很多促进宿主生长的细菌。[结论]饲料类型影响茶尺蠖幼虫的存活；饲料类型影响茶尺蠖幼虫肠道菌群结构。

Abstract:

[Objective] Ectropis obliqua Prout (Lepidoptera:Geometridae) is the most devastating insect pest of tea plants in China. Studying on the relationship among feed, gut microbial flora and fitness of Ectropis obliqua is of relatively high theoretical value for the management of E. obliqua. [Methods] We analyzed the effect of artificial diet (without tea ingredients) and tea leaves on survival of Ectropis obliqua larvae. We also analyzed the similarities and differences of gut microbial flora in Ectropis obliqua larvae fed with different feeds based on high-throughput sequencing technology. [Results] The mortality of larvae were significantly different. Larvae fed with artificial diet had high mortality. Larvae fed with artificial diet had higher bacterial diversity and evenness than larvae fed with tea leaves. Many bacteria were found in the gut of Ectropis obliqua larvae. [Conclusion] Feed affects the survival and the gut microbial flora of Ectropis obliqua larvae.