不同盐度胁迫下杜氏盐藻全转录组测序及注释

Gene expression profiling of Dunaliella salina under different salinity stress

摘要:

[目的]通过对杜氏盐藻的转录组进行测序和基因功能分析，阐明不同浓度盐胁迫对杜氏盐藻生长发育以及不同信号途径的影响。[方法]分别获取9% NaCl浓度和24% NaCl浓度培养下的杜氏盐藻转录组并通过Illumina平台进行测序。将所得的序列进行拼接、去冗余处理。[结果]获得40682个unigenes，其中注释到NR数据库的10905个，注释到NT数据库的2768个，注释到SWISS-PROT数据库的7261个，注释到COG/KOG数据库的6499个。受到高盐胁迫的杜氏盐藻细胞相比低盐环境下，有717个基因表达上调，1012个基因表达下调。进一步对60个显著差异基因进行了功能聚类，发现盐胁迫诱导了光合作用途径的基因表达。[结论]杜氏盐藻通过提高光合作用基因表达增强耐盐性。该研究最大范围上挖掘了杜氏盐藻在高盐和低盐环境的基因转录水平，为深入揭示杜氏盐藻盐胁迫下基因差异表达提供了平台，并为进一步研究杜氏盐藻耐盐机理提供理论依据。

Abstract:

[Objective] Through sequencing and genetic function analysis of the transcripts of Dunaliella salina, we studied its response to salt stress. [Methods] The transcriptome of Dunaliella salina under 9% and 24% NaCl concentration was obtained and sequenced by Illumina platform. All the unigenes were finally obtained by sequence splicing, redundancy removing based on the sequence clustering software. Functional notation and cluster analysis of unigenes were performed by comparing the unigenes to the database. [Results] A total of 40682 unigenes were obtained, including 10905 in the NR database, 2768 in the NT database, 7261 in the Swiss-prot database, and 6499 in the COG/KOG database. By comparing cells grown under hyper-and hyposaline conditions, 717 genes (41.46%) increased expression and 1012 genes (58.5%) decreased expression. Through the data integration analysis, 60 different expression genes of Dunaliella salina under salt stress were excavated. [Conclusion] Salinity stress up-regulated key genes in photosynthesis. Our findings provide reference for dissecting molecular mechanisms of salinity tolerance in algae and higher plants.