中国猪种布鲁氏菌分子流行病学特征分析
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内蒙古自治区自然科学基金(2018MS08004);传染病重大专项(2017ZX10303401);生物安全重点专项(2017YFC1200303,2016YFC1200701)


Molecular epidemiological characteristics of Brucella suis in China
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    摘要:

    [目的] 调查猪种布鲁氏菌的基因多态性和分子流行病学特征。[方法] 用经典分型方法对菌株的生物型进行鉴定,分析菌株的地理分布特点;用MLVA-16分型方法对60株猪种布鲁氏菌进行基因分型,采用在线软件评估分型方法的分辨率和位点的多态性,用BioNumerics 5.0软件进行聚类分析。[结果] 我国流行的猪种布鲁氏菌主要是猪种生物1型(33株)、2型(3株)和3型菌(24株);分布范围较广,包括广东、广西、内蒙古、北京、吉林、宁夏和西藏等地。MLVA-16分型方法对猪种布鲁氏菌具有极高的分辨力,多态性指数为0.992;Panel1、MLVA-11和Panel 2B均具有较高的分辨率,多态性指数分别为0.884、0.916和0.979。60株猪种布鲁氏菌聚为6大类52个基因型,5个共享基因型(GT24,GT25,GT26,GT28,GT29)包括13株布鲁氏菌,各基因型菌株间有潜在的流行病学关联,可能是分别来自相同传染源的暴发流行;另47株布鲁氏菌呈现独特的基因型,表明菌株来自无流行病学关联的零星散发病例。猪种布鲁氏菌的最小生成树表明我国菌株分别与美国、法国和波兰的菌株有完全相同的MLVA-15基因型。[结论] 中国猪种布鲁氏菌有较高的遗传多态性,并与美国、法国和阿根廷的菌株有较高的遗传相似性。我国猪种布病以零星散发为主。

    Abstract:

    [Objective] The aim of the present study was to investigate molecular epidemiology and genetic diversity characteristics of Brucella suis strains isolated from 14 different regions in China.[Methods] Biotypes of strains were discriminated by classical identification methods, both geographic distribution and etiology feature of B. suis were analyzed. Meanwhile, MLVA-16 genotyping assay was employed for genotyping of 60 B. suis strains. Diversity of each loci and resolution of genotyping assay were evaluated using online software.[Results] Our data indicated that three B. suis biovars (bv.1, 2, 3) were coexisted and strains numbers was 33, 2 and 24, respectively. Distribution of B. suis was wide and 14 provinces, Guangdong, Guangxi, Inner Mongolia, Beijing, Jilin, Ningxia, Xinjiang and Xizang were included. MLVA-16 approach is an excellent resolution for B. suis population, and diversity index of complete 16 loci was 0.992 based on Hunter-Gaston Discriminatory Index (HGDI). High resolution was observed in panel 1, MLVA-11 and panel 2b, their diversity index was 0.884, 0.916 and 0.979, respectively. Sixty B. suis strains were sorted into 6 clusters and formed 52 genotypes, 5 share (GT24,GT25, GT26, GT28 and GT29) genotypes including 13 isolates. The data indicated that these isolates have potential molecular epidemiology links, which maybe an outbreak from respective common source of infection. Remaining 47 genotypes being represented the single isolates, revealed that there also had unrelated and sporadic cases. A minimum spanning tree for B. suis using MLVA-15 revealed that China B. suis strains had complete identical MLVA-15 Genotypes with strains previously collected in America, France and Argentina.[Conclusion] B. suis strains of this study exhibited higher genetic diversity, as well as high genetic homology with strains obtained from America, France and Poland. The epidemic characterization of B. suis in China was sporadic.

    参考文献
    [1] Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. The Lancet Infectious Diseases, 2006, 6(2):91-99.
    [2] Zhang WY, Guo WD, Sun SH, Jiang JF, Sun HL, Li SL, Liu W, Cao WC. Human brucellosis, Inner Mongolia, China. Emerging Infectious Disease, 2010, 16(12):2001-2003.
    [3] Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. The New England Journal of Medicine, 2005, 352(22):2325-2336.
    [4] Hernández-Mora G, Bonilla-Montoya R, Barrantes-Granados O, Esquivel-Suárez A, Montero-Caballero D, González-Barrientos R, Fallas-Monge Z, Palacios-Alfaro JD, Baldi M, Campos E, Chanto G, Barquero-Calvo E, Chacón-Díaz C, Chaves-Olarte E, Guzmán Verri C, Romero-Zúñiga JJ, Moreno E. Brucellosis in mammals of Costa Rica:an epidemiological survey. PLoS ONE, 2017, 12(8):e0182644.
    [5] Whatmore AM, Dawson C, Muchowski J, Perrett LL, Stubberfield E, Koylass M, Foster G, Davison NJ, Quance C, Sidor IF, Field CL, St. Leger J. Characterisation of North American Brucella isolates from marine mammals. PLoS One, 2017, 12(9):e0184758.
    [6] Whatmore AM, Davison N, Cloeckaert A, Al Dahouk S, Zygmunt MS, Brew SD, Perrett LL, Koylass MS, Vergnaud G, Quance C, Scholz HC, Dick EJ Jr, Hubbard G, Schlabritz-Loutsevitch NE. Brucella papionis sp. nov., isolated from baboons (Papio spp.). International Journal of Systematic and Evolutionary Microbiology, 2014, 64(12):4120-4128.
    [7] Wiwanitkit V. Human brucellosis. Caspian Journal of Internal Medicine, 2016, 7(3):232.
    [8] Ran XH, Cheng JJ, Wang MM, Chen XH, Wang HX, Ge Y, Ni HB, Zhang XX, Wen XB. Brucellosis seroprevalence in dairy cattle in China during 2008-2018:a systematic review and meta-analysis. Acta Tropica, 2019, 189:117-123.
    [9] Sun MJ, Di DD, Li Y, Zhang ZC, Yan H, Tian LL, Jing ZG, Li JP, Jiang H, Fan WX. Genotyping of Brucella melitensis and Brucella abortus strains currently circulating in Xinjiang, China. Infection, Genetics and Evolution, 2016, 44:522-529.
    [10] Rahman AKMA, Saegerman C, Berkvens D, Melzer F, Neubauer H, Fretin D, Abatih E, Dhand N, Ward MP. Brucella abortus is prevalent in both humans and animals in Bangladesh. Zoonoses and Public Health, 2017, 64(5):394-399.
    [11] Tian GZ, Cui BY, Piao DR, Zhao HY, Li LY, Liu X, Xiao P, Zhao ZZ, Xu LQ, Jiang H, Li ZJ. Multi-locus variable-number tandem repeat analysis of Chinese Brucella strains isolated from 1953 to 2013. Infectious Diseases of Poverty, 2017, 6:89.
    [12] Olsen SC, Tatum FM. Swine brucellosis:current perspectives. Veterinary Medicine, 2017, 8:1-12.
    [13] Daugaliyeva A, Sultanov A, Usserbayev B, Baramova S, Modesto P, Adambayeva A, Acutis PL, Peletto S. Genotyping of Brucella melitensis and Brucella abortus strains in Kazakhstan using MLVA-15. Infection, Genetics and Evolution, 2018, 58:135-144.
    [14] Liu ZG, Di DD, Wang M, Liu RH, Zhao HY, Piao DR, Tian GZ, Fan WX, Jiang H, Cui BY, Xia XZ. MLVA genotyping characteristics of human Brucella melitensis isolated from Ulanqab of Inner Mongolia, China. Frontiers in Microbiology, 2017, 8:6.
    [15] Deng YM, Liu XX, Duan KF, Peng QS. Research progress on brucellosis. Current Medicinal Chemistry, 2018, doi:10.2174/0929867325666180510125009.
    [16] Bundle DR, McGiven J. Brucellosis:improved diagnostics and vaccine insights from synthetic glycans. Accounts of Chemical Research, 2017, 50(12):2958-2967.
    [17] Yang Y, Wang Y, Poulsen E, Ransburgh R, Liu XM, An BY, Lu NY, Anderson G, Wang CM, Bai JF. Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay. Scientific Reports, 2017, 7:1067.
    [18] Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems:an application of Simpson's index of diversity. Journal of Clinical Microbiology, 1988, 26(11):2465-2466.
    [19] Kang SI, Her M, Erdenebaataar J, Vanaabaatar B, Cho H, Sung SR, Lee JJ, Jung SC, Park YH, Kim JY. Molecular epidemiological investigation of Brucella melitensis circulating in Mongolia by MLVA16. Comparative Immunology, Microbiology and Infectious Diseases, 2017, 50:16-22.
    [20] Allen A, Breadon E, Byrne A, Mallon T, Skuce R, Groussaud P, Dainty A, Graham J, Jones K, Pollock L, Whatmore A. Molecular epidemiology of Brucella abortus in Northern Ireland-1991 to 2012. PLoS ONE, 2015, 10(9):e0136721.
    [21] Shang DQ, Xiao DL, Yin JM. Epidemiology and control of brucellosis in China. Veterinary Microbiology, 2002, 90(1/4):165-182.
    [22] Jiang H, Chen H, Chen JD, Tian GZ, Zhao HY, Piao DR, Cui BY. Genetic comparison of Brucella suis biovar 3 in clinical cases in China. Veterinary Microbiology, 2012, 160(3/4):546-548.
    [23] Mailles A, Ogielska M, Kemiche F, Garin-Bastuji B, Brieu N, Burnusus Z, Creuwels A, Danjean MP, Guiet P, Nasser V, Tourrand B, Valour F, Maurin M, O'Callaghan D, Mick V, Vaillant V, Jay M, Lavigne JP, de Valk H. Brucella suis biovar 2 infection in humans in France:emerging infection or better recognition? Epidemiology & Infection, 2017, 145(13):2711-2716.
    [24] Liu ZG, Wang LJ, Piao DR, Wang M, Liu RH, Zhao HY, Cui BY, Jiang H. Molecular investigation of the transmission pattern of Brucella suis 3 from Inner Mongolia, China. Frontiers in Veterinary Science, 2018, 5:271.
    [25] Kin MS, Fort M, de Echaide ST, Casanave EB. Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina. Veterinary Microbiology, 2014, 170(3/4):442-445.
    [26] Paolicchi FA, Terzolo HR, Campero CM. Isolation of Brucella suis from the semen of a ram. Veterinary Record, 1993, 132(3):67.
    [27] Mor SM, Wiethoelter AK, Lee A, Moloney B, James DR, Malik R. Emergence of Brucella suis in dogs in New South Wales, Australia:clinical findings and implications for zoonotic transmission. BMC Veterinary Research, 2016, 12(1):199.
    [28] Shevtsov A, Ramanculov E, Shevtsova E, Kairzhanova A, Tarlykov P, Filipenko M, Dymova M, Abisheva G, Jailbekova A, Kamalova D, Chsherbakov A, Tulegenov S, Akhmetova A, Sytnik I, Karibaev T, Mukanov K. Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16. Infection, Genetics and Evolution, 2015, 34:173-180.
    [29] Shevtsova E, Shevtsov A, Mukanov K, Filipenko M, Kamalova D, Sytnik I, Syzdykov M, Kuznetsov A, Akhmetova A, Zharova M, Karibaev T, Tarlykov P, Ramanculov E. Epidemiology of brucellosis and genetic diversity of Brucella abortus in Kazakhstan. PLoS ONE, 2016, 11(12):e0167496.
    [30] Jiang H, Fan MG, Chen JD, Mi JC, Yu RP, Zhao HY, Piao DR, Ke CW, Deng XL, Tian GZ, Cui BY. MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates. BMC Microbiology, 2011, 11:256.
    [31] Lai SJ, Zhou H, Xiong WY, Gilbert M, Huang ZJ, Yu JX, Yin WW, Wang LP, Chen QL, Li Y, Mu D, Zeng LJ, Ren X, Geng MJ, Zhang ZK, Cui BY, Li TF, Wang DL, Li ZJ, Wardrop NA, Tatem AJ, Yu HJ. Changing epidemiology of human brucellosis, China, 1955-2014. Emerging Infectious Disease, 2017, 23(2):184-194.
    [32] Garofolo G, Di Giannatale E, de Massis F, Zilli K, Ancora M, Cammà C, Calistri P, Foster JT. Investigating genetic diversity of Brucella abortus and Brucella melitensis in Italy with MLVA-16. Infection, Genetics and Evolution, 2013, 19:59-70.
    [33] Whatmore AM, Perrett LL, MacMillan AP. Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiology, 2007, 7:34.
    [34] Piao DR, Liu X, Di DD, Xiao P, Zhao ZZ, Xu LQ, Tian GZ, Zhao HY, Fan WX, Cui BY, Jiang H. Genetic polymorphisms identify in species/biovars of Brucella isolated in China between 1953 and 2013 by MLST. BMC Microbiology, 2018, 18(1):7.
    [35] Hanot Mambres D, Boarbi S, Michel P, Bouker N, Escobar-Calle L, Desqueper D, Fancello T, van Esbroeck M, Godfroid J, Fretin D, Mori M. Imported human brucellosis in Belgium:bio and molecular typing of bacterial isolates, 1996-2015. PLoS ONE, 2017, 12(4):e0174756.
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刘志国,王妙,赵鸿雁,朴东日,路殿英,李振军. 中国猪种布鲁氏菌分子流行病学特征分析[J]. 微生物学报, 2019, 59(11): 2107-2116

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  • 收稿日期:2018-11-22
  • 最后修改日期:2019-01-21
  • 在线发布日期: 2019-11-01
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