鼠衣原体改善DSS诱导的小鼠溃疡性结肠炎的作用研究

Amelioration of DSS-induced ulcerative colitis in mice by Chlamydia muridarum

摘要:

[目的] 探讨鼠衣原体（Chlamydia muridarum）对小鼠溃疡性结肠炎的作用。[方法] 取15只雌性C57BL/6J小鼠随机分为3组，每组5只动物，分别为空白对照组（Control）、肠炎模型组（DSS）、实验组（CM+DSS）。选取CM+DSS组小鼠予以2×10⁵IFU的鼠衣原体灌胃处理，并在其感染后第29天开始，给予DSS组和CM+DSS组的小鼠2% DSS饮水，持续5 d，每天监测小鼠体重和肠炎疾病评分，实验结束后检测小鼠结肠长度和结肠组织炎性改变。[结果] 肠炎模型组的小鼠均表现出典型的肠炎症状（包括体重减轻、肠炎疾病评分、结肠长度和组织炎性改变）；而经鼠衣原体预处理的小鼠（CM+DSS组）肠炎症状显著减轻，表现在肠炎疾病评分降低，体重和结肠长度有所恢复，肠组织炎性损伤减轻。[结论] 鼠衣原体对DSS诱导的小鼠溃疡性结肠炎具有改善作用。

关键词:鼠衣原体;肠道菌群;溃疡性结肠炎

Abstract:

[Objective] To study the effect of Chlamydia muridarum on the ulcerative colitis induced by dextran sulfate sodium (DSS) in mice. [Methods] In total 15 female C57BL/6J mice were randomly divided into 3 groups:control group, DSS group, experimental group (CM+DSS group). The mice of CM+DSS group were administrated with 2×10⁵ IFU Chlamydia muridarum by gavage. After 28 days, the mice of the DSS group and CM+DSS group drank 2% DSS water for 5 days, then they were monitored for body weight and disease activity index, the colon length of all mice, and the inflammatory damage of intestine tissue. [Results] The model of ulcerative colitis induced by DSS was well established with the body weight loss of colitis mice, increased disease activity index, short colon length, and severe inflammatory reaction of colon tissue. Compared with the DSS group, CM+DSS group recovered largely, including the body weight loss, disease activity index, colon length, and colon inflammatory damage. [Conclusion] Chlamydia muridarum could ameliorate ulcerative colitis induced by DSS.

Key words:Chlamydia muridarum;intestinal flora;ulcerative colitis