一株O型口蹄疫病毒在C57BL/6小鼠和原代细胞的传代及其全序的测定和分析

Passage of a type O foot-and-mouth disease virus in mice and cell and analysis of its complete sequence

摘要:

[目的] 旨在成功建立FMDV C57BL/6小鼠的实验感染模型。[方法] 采用体内和体外循环适应传代的方法，选取一株对C57BL/6小鼠不敏感FMDV O/HK/CHA/99 MF4，将其在C57BL/6小鼠（体内）和胎猪肾原代细胞FPK（体外）进行多次循环适应传代。[结果] 成功获得一株对C57BL/6小鼠敏感的FMDV O/HK/CHA/99 MF4C5株。[结论] 本研究成功建立了FMDV突变株感染C57BL/6小鼠的实验动物模型，为未来FMD疫苗效力的评估和致病性相关的研究奠定了基础。

关键词:口蹄疫病毒;小鼠;细胞;循环传代;全序的测定与分析

Abstract:

[Objective] To successfully establish the C57BL/6 mice experimental infection model for foot-and-mouth disease virus. [Methods] The foot-and-mouth disease virus O/HK/CHA/99 MF4 strain, which is insensitive to C57BL/6 mice, was selected and continuously passaged in C57BL/6 mice (in vivo) and fetal pig kidney cells (in vitro). [Results] We successfully obtained a foot-and-mouth disease virus mutant (O/HK/CHA/99 MF4C5), which was sensitive to C57BL/6 mouse. [Conclusion] This study successfully established C57BL/6 mice model for a foot-and-mouth disease virus mutant strain, which lays a foundation for the evaluation of the efficacy of foot-and-mouth disease vaccine and the study on pathogenicity in the future.

Key words:Foot-and-mouth disease virus;mice;cell;cyclic passage;analysis of complete sequence