益生菌Lactobacillus paracasei subsp.paracasei Zhang对海南地区部分青年人肠道菌群的影响

Effect of probiotics Lactobacillus paracasei subsp. paracasei Zhang on gut microbiota composition of young people living in Hainan

摘要:

[目的] 以海南地区征集的29名健康青年志愿者为研究对象，分析该地区青年人肠道菌群多样性，并探究益生菌Lactobacillus paracasei subsp.paracasei Zhang（LCZ）对其肠道菌群的影响。[方法] 29名健康青年志愿者每日补充2 g益生菌LCZ（0.5×10¹⁰ CFU/g），为期14 d。采集摄入益生菌LCZ第0、14、28天的志愿者粪便样品，采用PacBio SMRT测序技术基于16S rRNA基因全长测序分析志愿者粪便微生物组成和结构，评估益生菌LCZ对其肠道菌群的影响。[结果] 在门水平上，Firmicutes（54.46%）和Bacteroidetes（33.79%）在志愿者粪便微生物中含量最高；在属水平上，Bacteroides（23.13%）的相对含量最高；在种水平上，优势菌种为Faecalibacterium prausnitzii（9.72%）、Eubacterium rectale（7.00%）和Prevotella copri（6.07%）等。摄入益生菌LCZ 14 d后，肠道中的优势菌属变化不显著，低丰度菌属如Oscillospira和Parabacteroides等显著增加，Aeromonas和Fusicatenibacter等显著减少（P <0.05）。此外根据志愿者粪便中Lactobacillus含量的变化情况，将所有志愿者分为两组。其中一组志愿者在摄入LCZ后粪便中Lactobacillus菌属相对含量显著增加，同时该组志愿者肠道中其他菌种如Butyricicoccus pullicaecorum、Lactococcus raffinolactis和Parabacteroides distasoni亦显著增加；而另一组志愿者，Lactobacillus及上述菌种均未发生显著变化。[结论] 连续2周摄入益生菌LCZ对志愿者肠道菌群中优势菌属的相对丰度影响不显著，但对低丰度菌属的相对丰度影响显著。

Abstract:

[Objective] Twenty-nine healthy young volunteers from Hainan were selected as the research objects to analyze the diversity of gut microbes and to explore the effects of Lactobacillus paracasei subsp. paracasei Zhang on the fecal microbiota. [Methods] Volunteers were supplemented with 1×10¹⁰ CFU LCZ powders per day for 14 days. Fecal samples were collected on 0, 14 and 28 days. Microbial composition and structure of fecal were analyzed by PacBio SMRT. [Results] Firmicutes and Bacteroidetes were the most abundant phylum. Meanwhile, Bacteroides were the predominant genus. At the species level, the dominant bacteria species were Faecalibacterium prausnitzii, Eubacterium rectale and Prevotella copri. After taking LCZ for 14 days, the dominant bacteria did not change significantly, while low-abundance bacteria such as Oscillospira and Parabacteroides were significantly increased (P<0.05). In addition, volunteers were divided into two groups according to the changes of Lactobacillus. Butyricicoccus pullicaecorum, Lactococcus raffinolactis and Parabacteroides distasoni were significantly increased in samples of those with significantly increased content of Lactobacillus. Inversely, volunteers with no significant changes in the Lactobacillus content in their gut did not find similar changes. [Conclusion] Continuous consumption of probiotics LCZ for 2 weeks had no significant effect on the relative abundance of dominant bacteria in fecal microbiota of volunteers, while the low-abundance bacteria change significantly.