核桃壳提取液用于单针藻Monoraphidium sp.QLZ-3培养产油脂

Production of lipid from walnut shell extracts by Monoraphidium sp. QLZ-3

摘要:

[目的] 研究核桃壳提取液（walnut shell extracts，WSE）对单针藻Monoraphidium sp.QLZ-3生长和油脂积累的影响。[方法] 向BG-11培养基中添加不同量的WSE（培养基中保留有BG-11中全部营养成分）。[结果] 结果显示，当BG-11培养基中的WSE含量为40%时，单针藻的生物量产率及油脂产率达到（534.70±4.07）mg/（L·d）和（296.35±15.36）mg/（L·d），相比对照组分别提高了的14.82%和33.50%，蛋白质和碳水化合物含量分别有不同程度的上调和下调。与对照组相比，微藻中谷胱甘肽（glutathione，GSH）和超氧化物歧化酶（superoxide dismutase，SOD）含量与活性均上调。此外，WSE作用下，微藻对多酚的移除达到84.37%，同时上调了核酮糖1，5-二磷酸羧化酶基因（ribulose 1，5-bisphosphate carboxylase/oxygenase，rbcL）和乙酰辅酶A羧化酶（acetyl coenzyme A carboxylase，accD）基因的表达量。[结论] 研究表明，WSE联合BG-11可以提高微藻的生物量产率和油脂产率，降低微藻培养的原料成本，为核桃壳的资源化利用及微藻的工业化生产提供了一定的技术支撑。

Abstract:

[Objective] The effects of walnut shell extracts (WSE) on the growth and lipid accumulation of Monoraphidium sp. QLZ-3 were explored. [Methods] Different volumes of WSE were added to BG-11 medium (all nutrients in BG-11 were retained in the medium). [Results] The biomass and lipid productivity reached (534.7±4.07) mg/(L·d) and (296.4±15.36) mg/(L·d) with addition of 40% WSE in the culture medium, increasing 14.82% and 33.50%, respectively, with upregulation of protein and downregulation of carbohydrate. [Conclusion] Exogenous WSE could enhance the biomass and lipid productivities in microalgae.