细菌糖基化基本元件及其应用研究进展

Fundamental elements of bacterial glycosylation system

摘要:

近年来，细菌糖基化修饰系统的研究受到了越来越广泛的关注。已有文献报道了诸多细菌糖基化修饰系统，包括最具有代表性的空肠弯曲杆菌的N-糖基化修饰系统以及脑膜炎奈瑟菌的O-糖基修饰系统。本文在已有的研究基础上进行了系统的归纳总结，讨论对细菌蛋白质糖基化系统的理解，同时综述了细菌蛋白质糖基化应用方面的相关进展。

关键词:糖基化;原核表达系统;糖基转移酶;重组疫苗

Abstract:

In recent years, glycosylation modification system in bacteria has attracted more and more attention in both academic and industrial fields. Numerous glycosylation modification systems in bacteria have been reported, the most representative cases are N-glycosylation modification system in Campylobacter jejuni and O-glycosylation modification system in Neisseria meningitidis. This review systematically summarizes the glycosylation modification system in bacteria to better understand this system and enlists the situation of the application using the protein glycosylation modification system in bacteria. At the same time, we provide some theoretical knowledges and examples for the design of virus-like particle-based vaccines where glycosylation is required.

Key words:glycosylation;prokaryotic expression system;glycosyltransferases;recombinant vaccine