紫色红曲霉Mp-21胞内次级代谢产物的分离纯化与活性研究

Separation, purification and bioactivity analysis of intracellular secondary metabolites from Monascus purpureus Mp-21

摘要:

[目的] 紫色红曲霉（Monascus purpureus） Mp-21胞内次级代谢产物的分离纯化与活性研究。[方法] 通过综合运用多种色谱分离方法对次级代谢产物进行分离纯化，最后通过多种图谱数据对化合物进行结构的解析与鉴定。对分离纯化得到的单体化合物进行抗氧化与降血糖相关酶抑制活性的测定。[结果] 从紫色红曲霉Mp-21胞内分离纯化并鉴定出3个活性化合物baicalein （1）、genistein （2）、glycitein （3），其中化合物1为首次从红曲菌科中发现，化合物2和3为首次从紫色红曲霉中发现。在体外抗氧化和抑制酶活性试验中，化合物1对1，1-二苯基-2-三硝基苯肼（DPPH）、O^2–、OH^–自由基具有较强的清除能力，对α-葡萄糖苷酶和α-淀粉酶也具有较强的抑制作用，IC₅₀分别为5.67、17.46、36.40、30.94、430.93 μg/mL。[结论] 紫色红曲霉Mp-21具有开发成抗氧化、降血糖等功能性食品的潜力。

Abstract:

[Objective] The active components of intracellular secondary metabolites from Monascus purpureus Mp-21 were studied. [Methods] The intracellular secondary metabolites were separated and purified by a variety of chromatographic separation methods, and the structure of the compounds was analyzed and identified using a variety of chromatographic data. Monomeric compounds were tested for antioxidant activity and hypoglycemia-related enzyme inhibitory activity. [Results] Three active compounds, namely baicalein, genistein and glycitein were isolated and identified. Baicalein was first discovered from Monascaceae whereas genistein and glycitein were first discovered from Monascus purpureus. In vitro antioxidant activity test and hypoglycemic- related enzyme activity test, baicalein had strong scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH), O^2- and OH^- radical, and strong inhibitory effect on α-glucosidase and α-amylase, with IC₅₀ of 5.67, 36.40, 17.46, 30.94, 430.93 μg/mL, respectively. [Conclusion] Secondary metabolites from Monascus purpureus Mp-21 have the potential to be developed into functional food such as antioxidant and hypoglycemic agents.