非小细胞肺癌患者肠道微生物特征分析

Analysis on characteristics of microbiome structure in non-small cell lung cancer patients

摘要:

[目的] 分析非小细胞肺癌患者与肺部良性病变患者肠道微生物的构成差异，探究特异肠道菌群对非小细胞肺癌发生发展的影响。[方法] 收集63例患者粪便样本，非小细胞肺癌患者39例，其中肺腺癌（ADC）32例，肺鳞癌（SCC）7例，肺部良性病变患者24例，进行16S rDNA测序。[结果] 毛螺菌属（Lachnospira）、瘤胃球菌属（Ruminococcus）、粪杆菌属（Faecalibacterium）、罗氏菌属（Roseburia）和纺锤链杆属（Fusicatenibacter）在肺腺癌患者中显著富集，在肺鳞癌和肺部良性病变中无明显差异。嗜血杆菌属（Haemophilus）、普雷沃菌属（Prevotella）、链球菌属（Streptococcus）和柠檬酸杆菌属（Citrobacter）在肺部良性病变患者肠道中显著富集。[结论] 非小细胞肺癌患者与肺部良性病变患者肠道中均存在特异的微生物菌群。

Abstract:

[Objective] To describe and compare the structure of intestinal microbiota between patients with non-small cell lung cancer and benign lung disease and explore the influence of specific microbial flora in the lung cancer.[Methods] We collected 63 cases of fecal samples which including 39 non-small cell lung cancer patients and 24 benign lung disease patients and use high-throughput second-generation sequencing technology platform to analyze the V3-V4 region of 16S rDNA gene in fecal samples of bacteria to explore the overall structural characteristic of intestinal microbiota.[Results] Lachnospira, Ruminococcus, Faecalibacterium, Roseburia, Fusicatenibacter are significantly enriched in lung adenocarcinoma patients. But in lung squamous cell carcinoma and benign lesions there is no obvious difference. And Haemophilus, Prevotella, Streptococcus, Citrobacter are significantly enriched in the intestines of patients with benign diseases.[Conclusions] Non-small cell lung cancer and benign diseases have different gut microbiome.