2025年6月13日 周五
首页 > 过刊浏览>2021年第61卷第10期 >2999-3009. DOI:10.13343/j.cnki.wsxb.20200780
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基于环介导等温扩增技术的微流控芯片在病原菌检测方面的研究进展
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基金项目:

国家重点研发计划（2017YFC1601200）


Advances in the detection of pathogenic bacteria by using loop-mediated isothermal amplification-based microfluidic chip
Author:
  • Yuting Shang

    Yuting Shang

    Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, Guangdong Province, China;State Key Laboratory of Food Science and Technology, National Engineering Research Centre for Functional Food, Synergetic Innovation Centre of Food Safety and Quality Control, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China
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  • Qinghua Ye

    Qinghua Ye

    Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, Guangdong Province, China
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  • Shuzhen Cai

    Shuzhen Cai

    Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, Guangdong Province, China
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  • Qingping Wu

    Qingping Wu

    Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, Guangdong Province, China
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  • Jumei Zhang

    Jumei Zhang

    Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, Guangdong Province, China
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  • 摘要
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    摘要:

    环介导等温扩增（LAMP）技术是一种新兴的核酸恒温扩增技术，与微流控芯片技术相结合，可实现对病原菌的快速检测，具有特异性强、灵敏度高、操作简单等优点。本文根据不同终产物的检测方法对目前检测病原菌的相关微流控LAMP芯片进行了分类与介绍，并对技术的改进和存在的问题进行了分析，以期为后续的相关研究提供参考。

    Abstract:

    Loop-mediated isothermal amplification (LAMP) is an emerging nucleic acid amplification technique under isothermal condition, which can combined with microfluidic chip to rapidly detect the pathogenic bacteria. It is of higher specificity, higher sensitivity and simpler operation. This article classified and introduced the LAMP-based microfluidic chip for the detection of pathogenic bacteria, based on the analysis of different end-point detection methods. And also, we analyzed the improvement and existing problems of this technology, which can provide great reference value for the further studies and industry development.

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尚玉婷,叶青华,蔡淑珍,吴清平,张菊梅. 基于环介导等温扩增技术的微流控芯片在病原菌检测方面的研究进展[J]. 微生物学报, 2021, 61(10): 2999-3009

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  • 收稿日期:2020-12-22
  • 最后修改日期:2021-01-20
  • 在线发布日期: 2021-09-29
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