硒与内生真菌对中华羊茅生长和代谢产物的影响

Effects of Se and Epichloë endophyte on the growth and metabolic compounds of Festuca sinensis

摘要:

[目的] 探讨亚硒酸钠和内生真菌对中华羊茅生长和代谢产物的影响。[方法] 采用气相色谱-质谱联用仪检测中华羊茅两个地理种群带菌（E+）和不带菌（E-）植株地上部分和根系的非靶标代谢产物，利用单因素方差分析（LSD）显著性统计挖掘处理间的差异性代谢产物。[结果] 从地理种群海北（HB）和玉树（YS）植株中分别鉴定出206种和205种化合物，地上部分和根系中代谢产物存在较大的差异性，根据显著性统计检验P<0.001分别从地理种群HB地上部分、HB根系、地理种群YS地上部分、YS根系筛选出差异性代谢物27种、42种、40种、33种，这些差异性代谢物主要是一些含氮化合物、碳水化合物和有机酸。HB地上部分与HB根系共同差异性代谢物9种，YS地上部分与YS根系共同差异性代谢物8种，HB地上部分与YS地上部分共同差异性代谢物5种，HB根系与YS根系共同差异性代谢物14种。地理种群HB内生真菌极显著增加植物地上部分和根系中tyrose含量（P<0.001），加硒促进E+和E-地上部分和根系α-ketoisocaproic acid含量极显著上升（P<0.001），加硒和内生真菌极显著积累地上部分和根系aspartic acid含量（P<0.001）。加硒极显著提高地理种群YS E+和E-地上部分和根系α-ketoisocaproic acid和acetol含量（P<0.001）。[结论] 中华羊茅两个地理种群地上部分和根系非极性代谢物存在较大的差异性，亚硒酸钠和/或内生真菌能提高一些代谢产物的含量。

关键词:中华羊茅;地理种群;内生真菌Epichloë sp.;硒;代谢分析

Abstract:

[Objective] To investigate the effects of Na₂SeO₃ and Epichloë sp. on Festuca sinensis, we studied the growth indexes and metabolic compounds. [Methods] By using gas chromatography-mass spectrometer, we detected the metabolic compounds of the shoots and roots of F. sinensis two geographical populations with and without Epichloë endophyte, and metabolite differences were determined among treatments using LSD test. [Results] Geographical populations Haibei (HB) and Yushu (YS) respectively had 206 metabolites and 205 metabolites. There were significant differences in metabolites between shoots and roots. Geographical population HB shoots, HB roots, YS shoots and YS roots, respectively showed 27, 42, 40 and 33 marked metabolites using LSD (P<0.001). These marked metabolites were some nitrogen compounds, carbohydrates and organic acids. 9, 8, 5 and 14 common marked metabolites were respectively found between HB shoots and roots, between YS shoots and roots, between HB and YS shoots, and between HB and YS roots. Epichloë endophyte caused a highly significant increase in tyrose for geographical population HB shoots and roots (P<0.001). Selenium induced highly significant increases in α-ketoisocaproic acid in HB E+ and E-shoots and roots (P<0.001). There were highly significant increases of aspartic acid for HB shoots and roots by interaction of the Se and Epichloë endophyte (P<0.001). In addition, selenium treatment highly significantly increased α-ketoisocaproic acid and acetol for shoots and roots in geographical population YS E+ and E-(P<0.001). [Conclusion] There were significant differences in metabolites of the shoots and roots of two geographical populations. Epichloë endophyte infection and/or selenium treatment can increase the concentrations of some compounds.

Key words:Festuca sinensis;geographical population;Epichloë endophyte;selenium;metabolite analysis