果实红色与黄色番茄品种植株茎部内生细菌群落结构及代谢功能特征

Characteristics of endophytic bacterial community structure and metabolic function in stems of tomatoes between red and yellow varieties

摘要:

【目的】比较果实颜色分别为红色和黄色的番茄植株茎部内生细菌群落组成及代谢功能特征，旨在探究番茄果色形成与植株内生细菌的关联，为构建番茄育种新型的评价体系，以及开发利用有益微生物功能提供理论依据和技术支撑。【方法】基于MiSeq高通量测序技术，分析果实颜色分别为红色和黄色番茄品种植株茎部内生细菌群落组成及相关代谢功能生物信息学分析。【结果】果实颜色分别为红色和黄色番茄品种植株茎部中，短状杆菌属（Brachybacterium）、未分类大肠杆菌属（unclassified_f_Enterobacteriaceae）、乳酸杆菌属（Lactobacillus）、棒杆菌属（Corynebacterium）、绿脓杆菌属（Pseudomaonas）、微杆菌属（Microbacterium）、norank_f_norank_o_Gaiellales、褚氏杆菌属（Chujaibacter）、鞘氨醇单胞杆菌属（Sphigomonas）细菌是红色番茄品种植株茎部特有的优势内生细菌属；短小杆菌属（Curtobacterium）、norank_f_Mitochondria、拟无枝酸菌属（Amycolatopsis）和束毛球菌属（Trichococcus）是黄色番茄品种植株茎部特有的优势的内生细菌属。此外，果实颜色为红色的番茄品种植株茎部中，拥有比黄色番茄品种更为丰富的内生细菌属与基因功能。【结论】果实颜色分别为红色和黄色的番茄品种植株茎部，形成了特异的内生细菌群落组成与功能，红色品种拥有比黄色品种更丰富的特有优势内生细菌属与基因功能，番茄果实颜色形成与植株内生细菌的群落组成与功能紧密相关。

Abstract:

[Objectives] To provide theoretical basis and technical support for exploiting and utilizing beneficial microbes and their functions, the mechanism of color formation in tomato fruits related to endophytic bacteria in stems of different tomato varieties were analyzed. [Methods] Based on MiSeq high-throughput sequencing technology, informatics analysis of the endophytic bacteria in stems of tomatoes between red and yellow varieties were conducted. [Results] At the genus level, Brachybacterium, unclassified_f_Enterobacteriaceae, Lactobacillus, Corynebacterium, Pseudomaonas, Microbacterium, norank_f_norank_o_Gaiellales, Chujaibacter, Sphigomonas were the specific dominant endophytic bacteria in stems of red tomato varieties; And Curtobacterium, norank_f_Mitochondria, Amycolatopsis and Trichococcus were the specific dominant endophytic bacteria in stems of yellow tomato varieties. Meanwhile, the abundance of endophytic bacteria and their functions in stems of red tomato varieties were higher than those of yellow tomato varieties. [Conclusions] The composition of endophytic bacteria in stems between with red and yellow fruits of tomato varieties formed specific dominant endophytic bacteria, respectively. And higher abundance of specific endophytic bacterial genera and gene functions were found in red fruit tomato varieties than those of the yellow variety. It suggests that the fruit color formations were significantly related to the composition and function of endophytic bacteria in plant.