嗜酸乳杆菌La28和植物乳杆菌LP45对过敏小鼠的干预作用

Anti-allergic effects of Lactobacillus acidophilus La28 and L. plantoplantum LP45 in mice

摘要:

【目的】研究嗜酸乳杆菌La28和植物乳杆菌LP45对特应性皮炎和过敏性哮喘小鼠的干预作用，解析其在相关免疫调节上的菌株特异性差异。【方法】对特应性皮炎研究中将40只小鼠随机分为对照组、模型组、La28组和LP45组，除对照组外的其他三组采用2，4-二硝基氟苯诱导耳肿胀和皮炎模型，La28组和LP45组每天灌胃5×10⁸ CFU/mL相应菌粉溶液持续3周，测定各组耳肿胀率并进行皮损症状评分，观察皮肤组织的病理变化，检测皮肤组织中IL-4和IL-13含量。过敏性哮喘研究中除对照组外的其他3组采用卵清蛋白和氢氧化铝佐剂诱导哮喘模型，La28组和LP45组每天灌胃5×10⁸ CFU/mL相应菌粉溶液持续4周，评价各组受试小鼠哮喘症状，观察肺组织病理变化，检测肺组织中IL-4、IL-13和IL-17含量。【结果】La28和LP45可显著降低特应性皮炎小鼠耳肿胀和皮肤损伤，并显著降低IL-4的分泌；对IL-13分泌水平呈下降趋势但与模型组没有显著差异。La28和LP45可缓解小鼠的哮喘症状，LP45可减轻肺组织的病理损伤，减少炎症细胞浸润，肺组织中IL-4、IL-13和IL-17含量显著降低；La28对IL-13分泌量显著降低，IL-4和IL-17分泌水平呈下降趋势但差异不显著。【结论】2株乳酸杆菌尤其是植物乳杆菌LP45，可特异性地调节小鼠的免疫机能和改善组织损伤，从而发挥对特应性皮炎和哮喘的缓解作用。

关键词:过敏;乳酸杆菌;皮炎;哮喘;细胞因子

Abstract:

[Objective] To compare the allergic effects of Lactobacillus acidophilus La28 and L. plantoplantum LP45 against allergic dermatitis and allergic asthma in mice, and to analyze the strain-specific differences in related immune regulatory effects.[Methods] In order to study atopic dermatitis, we randomly assigned 40 mice into control group, model group, La28 group, and LP45 group. Except those in the control group, the mice in other three groups were treated with 2,4-dinitrofluorobenzene to induce ear swelling and dermatitis. The mice in La28 group and LP45 group received 5×10⁸ CFU/mL corresponding bacteria by intragastric administration every day for three weeks. We then measured the ear swelling rate and skin lesion score in each group, observed the pathological changes of skin, and determined the levels of interleukin-4 (IL-4) and interleukin-13 (IL-13) in the skin. To study allergic asthma, we treated mice in the other three groups except the control group with ovalbumin and aluminum hydroxide adjuvant to induce asthma. The mice in La28 group and LP45 group were fed with 5×10⁸ CFU/mL corresponding bacteria every day for four weeks. We then evaluated the asthma symptoms of mice in each group, examined the pathological changes in the lung tissue, and determined the levels of IL-4, IL-13, and interleukin-17 (IL-17) in the lung tissue.[Results] La28 and LP45 significantly relieved ear swelling and skin damage, significantly reduced IL-4 secretion, and slightly lowered the IL-13 level in the mice with allergic dermatitis. La28 and LP45 alleviated asthma symptoms. LP45 mitigated the pathological injury of the lung tissue, reduced inflammatory cell infiltration, and significantly down-regulated the levels of IL-4, IL-13, and IL-17 in the lung tissue. La28 significantly reduced the IL-13 secretion while insignificantly lowered the levels of IL-4 and IL-17.[Conclusion] The two Lactobacillus strains, especially LP45, can regulate the immune function and repair the damaged tissues to relieve atopic dermatitis and asthma in mice.

Key words:allergy;Lactobacillus;dermatitis;asthma;cytokines