葛仙米多糖对高脂小鼠血脂和肠道微生物的影响

Effects of Nostoc sphaeroids Kütz polysaccharide on blood lipid and gut microflora in high-fat diet mice

摘要:

【目的】研究葛仙米多糖对高脂饲料喂养小鼠血脂和肠道微生物的影响。【方法】将健康的8周龄雄性小鼠分成5组，每组10只：正常组C57/6CNC小鼠（N：灌胃生理盐水，喂饲标准饲料），对照组ApoE^-/-小鼠（C：灌胃生理盐水，喂饲标准饲料），模型组ApoE^-/-小鼠（M：灌胃生理盐水，喂饲高脂高胆固醇饲料），葛仙米多糖低剂量组ApoE^-/-小鼠（L：灌胃葛仙米多糖0.4 g/kg BW，喂饲高脂高胆固醇饲料）以及葛仙米多糖高剂量组ApoE^-/-小鼠（H：灌胃葛仙米多糖0.8 g/kg BW，喂饲高脂高胆固醇饲料）。试验为期22周。试验结束后，采集血清、肝脏和结肠组织、盲肠内容物样本利用生化分析仪测定血脂含量，HE染色观察组织结构，油红O染色观测肝脏脂肪沉积程度，16S rRNA基因高通量测序和生物信息处理技术分析盲肠内容物中肠道菌群的多样性和组成结构。【结果】葛仙米多糖降低了高脂高胆固醇饲料喂养小鼠的血脂，减少了肝脏组织的脂肪沉积，改善了肠道微生物的多样性和丰富度。【结论】膳食给予葛仙米多糖可改善高脂小鼠的脂代谢，调节肠道微生态平衡。

关键词:葛仙米多糖;高脂饮食;肠道菌群

Abstract:

[Objective] To study the effects of Nostoc sphaeroids Kütz polysaccharide (NSKP) on blood lipid and gut microflora in high fat diet mice.[Methods] The healthy 8-week-old male mice were assigned into 5 groups, with 10 mice in each group. The normal group included the C57/6CNC mice administrated with normal saline by gavaged with standard diet, and the control group consisted of ApoE^-/- mice administrated with normal saline by gavaged with standard diet. The model group consisted of the ApoE^-/- mice administrated with normal saline by gavage and fed with high-fat and high-cholesterol diet. The ApoE^-/- mice in the low-dose and high-dose NSKP groups were fed with high-fat and high-cholesterol diet and received 0.4 g/kg and 0.8 g/kg BW NSKP, respectively. The experiment lasted for 22 weeks, and then the serum, liver, colon tissue, and cecal contents samples were collected from the mice. Serum lipid level was measured by the biochemical analyzer. The tissue structure and fat deposition were observed via HE staining and oil red O staining, respectively. The diversity and composition of gut microflora in cecal contents were analyzed by 16S rRNA gene high-throughput sequencing and bioinformatics tools.[Results] NSKP reduced the serum lipid level and fat deposition in liver tissue, and improved the diversity and relative abundance of gut microflora in high-fat diet mice.[Conclusion] NSKP can improve lipid metabolism and regulate gut microecological balance in high-fat diet mice.

Key words:Nostoc sphaeroids Kü;tz polysaccharide;high-fat diet;gut microflora