弱后酸化瑞士乳杆菌的筛选及发酵特性研究
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Isolation and characterization of a Lactobacillus helveticus strain with reduced post-acidification
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    摘要:

    【目的】在大健康背景下,发酵乳凭借其优良的营养和保健功能备受青睐。后酸化是发酵乳在贮存、运输和销售等过程中活性乳酸菌继续代谢产酸导致的,严重影响产品的感官品质,降低菌株的存活率。本文通过筛选弱后酸化的瑞士乳杆菌,改善发酵乳的后酸化问题。【方法】本研究以瑞士乳杆菌L551为出发菌株,硫酸新霉素为筛选压力,筛选弱后酸化的瑞士乳杆菌。以鲜牛奶为基料,比较弱后酸化菌株与原始菌株发酵特性之间的差异。【结果】筛选得到了弱后酸化瑞士乳杆菌L551-1,其发酵过程产酸变慢,42℃发酵7-8 h产酸速度为9 °T/h,较原始菌株(19 °T/h)降低53%;42℃发酵24 h酸度为155 °T,较原始菌株(259 °T)降低40%;发酵乳4℃存储21 d的酸度为122 °T,较原始菌株(158 °T)降低23%。采用气相色谱-质谱联用仪(gas chromatography-mass spectrometry,GC-MS)比较两株菌发酵乳的挥发性风味物质组成和含量,未见显著差异。【结论】通过硫酸新霉素筛选获得的弱后酸化瑞士乳杆菌L551-1可显著改善后酸化问题,这对直投式发酵剂的开发与应用具有重要意义。

    Abstract:

    [Objective] Fermented dairy are favored for their excellent nutrition and health functions concomitantly. Post-acidification is caused by the continuous metabolism of active lactic acid bacteria during the process of storage, transportation and sales, which seriously affects its sensory quality and the number of viable strains. This study screened the low-post-acidification Lactobacillus helveticus to resolve the post-acidification of its fermented dairy.[Methods] In this study, Lactobacillus helveticus L551 was used as the original strain. Using neomycin sulfate as the selection pressure select the weakly acidified Lactobacillus helveticus. Comparing the difference of fermentation characteristics between the weakly acidified strain and the original strain.[Results] The acid production rate of weakly acidified Lactobacillus helveticus L551-1 was slowed down. The acid production rate of 7-8 h at 42℃ was 9 °T/h, which was 53% lower than the original strain (19 °T/h); the acidity of fermentation at 42℃ for 24 h was 155 °T, which was 40% lower than that of the original strain (259 °T); the post-acidification of fermented milk was 122 °T stored at 4℃ for 21 days, which was 23% lower than the original strain (158 °T). Using gas chromatography-mass spectrometry (GC-MS) compared the composition and type of volatile flavor compounds in fermented milk of the two strains, and there was no significant difference.[Conclusion] Through neomycin sulfate, the weakly post-acidified Lactobacillus helveticus L551-1 was obtained, which can significantly improve the post-acidification problem of fermented milk, and have a great significance to the development and application of direct starter.

    参考文献
    [1] Ghosh T, Beniwal A, Semwal A, Navani NK. Mechanistic insights into probiotic properties of lactic acid bacteria associated with ethnic fermented dairy products. Frontiers in Microbiology, 2019, 10:502.
    [2] 于洁, 张和平. 益生菌发酵乳的研究及产业化进展. 中国食品学报, 2020, 20(10):1-7. Yu J, Zhang HP. Research and industrialization progress on probiotics fermented dairy products. Journal of Chinese Institute of Food Science and Technology, 2020, 20(10):1-7. (in Chinese)
    [3] Guan CR, Chen X, Zhao RF, Yuan Y, Huang XY, Su JB, Ding XL, Chen X, Huang YJ, Gu RX. A weak post-acidification Lactobacillus helveticus UV mutant with improved textural properties. Food Science & Nutrition, 2021, 9(1):469-479.
    [4] 张俊桃, 刘文俊, 孟和毕力格. 酸奶弱后酸化发酵剂乳酸菌育种技术研究进展. 中国微生态学杂志, 2017, 29(4):481-486. Zhang JT, Liu WJ, Meng H. Breeding of lactic acid bacteria with low post-acidification in yoghurt:progress in research. Chinese Journal of Microecology, 2017, 29(4):481-486. (in Chinese)
    [5] Ho SW, El-Nezami H, Shah NP. The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection. Scientific Reports, 2020, 10:499.
    [6] Hassan MU, Nayab H, Shafique F, Williamson MP, Almansouri TS, Asim N, Shafi N, Attacha S, Khalid M, Ali N, Akbar N. Probiotic properties of Lactobacillus helveticus and Lactobacillus plantarum isolated from traditional Pakistani yoghurt. BioMed Research International, 2020, 2020:8889198.
    [7] Myles EM, O'Leary ME, Smith R, MacPherson CW, Oprea A, Melanson EH, Tompkins TA, Perrot TS. Supplementation with combined Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 across development reveals sex differences in physiological and behavioural effects of western diet in long-Evans rats. Microorganisms, 2020, 8(10):1527.
    [8] Moser A, Wüthrich D, Bruggmann R, Eugster-Meier E, Meile L, Irmler S. Amplicon sequencing of the slpH locus permits culture-independent strain typing of Lactobacillus helveticus in dairy products. Frontiers in Microbiology, 2017, 8:1380.
    [9] Papadimitriou K, Alegría Á, Bron PA, De Angelis M, Gobbetti M, Kleerebezem M, Lemos JA, Linares DM, Ross P, Stanton C, Turroni F, Van Sinderen D, Varmanen P, Ventura M, Zúñiga M, Tsakalidou E, Kok J. Stress physiology of lactic acid bacteria. Microbiology and Molecular Biology Reviews, 2016, 80(3):837-890.
    [10] 张祥. 低H+-ATPase活性植物乳杆菌突变菌的筛选及其调控机制的研究. 浙江科技学院硕士学位论文, 2017.
    [11] Permata YM, Bachri M, Reveny J, Sibuea FM. Formulation and quantitative analysis of betamethasone valerate and neomycin sulfate cream by high performance liquid chromatography and spectrophotometry. Open Access Macedonian Journal of Medical Sciences, 2019, 7(22):3841-3846.
    [12] 孙自顶. 植物乳杆菌的诱变选育及其在酸奶中的应用. 齐鲁工业大学硕士学位论文, 2016.
    [13] 刘飞, 杜鹏, 王玉堂, 刘芳, 霍贵成. 保加利亚乳杆菌H+-ATPase缺陷型菌株的筛选. 微生物学报, 2009, 49(1):38-43. Liu F, Du P, Wang YT, Liu F, Huo GC. Screening of H+-ATPase deficient mutant of Lactobacillus delbrueckii subsp. bulgaricus. Acta Microbiologica Sinica, 2009, 49(1):38-43. (in Chinese)
    [14] 马霞, 张柏林. 乳酸菌H+-ATPase延缓酸奶后酸化的应用研究. 乳业科学与技术, 2008, 31(5):215-218. Ma X, Zhang BL. Applied study on H+-ATPase of lactic acid bacteria to postpone post-acidification of yogurt. Journal of Dairy Science and Technology, 2008, 31(5):215-218. (in Chinese)
    [15] Koebmann BJ, Nilsson D, Kuipers OP, Jensen PR. The membrane-bound H(+)-ATPase complex is essential for growth of Lactococcus lactis. Journal of Bacteriology, 2000, 182(17):4738-4743.
    [16] Tangyu MZ, Muller J, Bolten CJ, Wittmann C. Fermentation of plant-based milk alternatives for improved flavour and nutritional value. Applied Microbiology and Biotechnology, 2019, 103(23/24):9263-9275.
    [17] Dan T, Wang D, Wu SM, Jin RL, Ren WY, Sun TS. Profiles of volatile flavor compounds in milk fermented with different proportional combinations of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Molecules, 2017, 22(10):1633.
    [18] O'Sullivan E, Condon S. Relationship between acid tolerance, cytoplasmic pH, and ATP and H+-ATPase levels in chemostat cultures of Lactococcus lactis. Applied and Environmental Microbiology, 1999, 65(6):2287-2293.
    [19] Kim SY, Hyeonbin O, Lee P, Kim YS. The quality characteristics, antioxidant activity, and sensory evaluation of reduced-fat yogurt and nonfat yogurt supplemented with basil seed gum as a fat substitute. Journal of Dairy Science, 2020, 103(2):1324-1336.
    [20] 宋慧敏. 热处理对牛乳风味及保藏品质的影响. 东北农业大学硕士学位论文, 2015.
    [21] 马媛, 耿伟涛, 王金菊, 王艳萍. 乳酸菌代谢与食品风味物质的形成. 中国调味品, 2019, 44(1):159-163, 172. Ma Y, Geng WT, Wang JJ, Wang YP. Lactic acid bacteria metabolism and formation of food flavor substances. China Condiment, 2019, 44(1):159-163, 172. (in Chinese)
    [22] Ekinci FY, Gurel M. Effect of using propionic acid bacteria as an adjunct culture in yogurt production. Journal of Dairy Science, 2008, 91(3):892-899.
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杨玲,仵红岩,贾洪利,张彦位,孙策,路江浩,何方,齐世华. 弱后酸化瑞士乳杆菌的筛选及发酵特性研究[J]. 微生物学报, 2022, 62(3): 824-835

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  • 收稿日期:2021-08-27
  • 最后修改日期:2021-10-18
  • 在线发布日期: 2022-03-07
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