乳酸乳球菌表达系统的发展现状与前景展望

Development and prospects of Lactococcus lactis expression system

摘要:

乳酸乳球菌作为全球公认安全的微生物，具有多种益生作用，常被用作基因工程宿主菌。在过去的二十年中，乳酸乳球菌作为载体在递呈病毒、细菌抗原等方面得到了广泛的应用，并且在不同领域发挥着重要作用。本文以乳链菌肽控制的表达（nisin-controlled expression，NICE）系统为例，介绍了基于乳酸乳球菌的表达系统的组成、不同组分的功能特点以及该表达系统在食品、疫苗和畜牧兽医等领域的应用，总结分析了NICE系统的特点以及需要改进的地方，并对该表达系统未来的发展前景进行了展望。

关键词:乳酸乳球菌;宿主菌;乳链菌肽控制的系统;应用

Abstract:

Lactococcus lactis has a variety of probiotic effects and is often used as a host strain for genetic engineering. It is generally recognized as safe (GRAS) by the Food and Drug Administration (FDA). In the past two decades, L. lactis has been widely used as a carrier in presenting viruses and bacterial antigens, playing an important role in different fields. In this review, we introduced the nisin-controlled expression (NICE) system from the aspects of composition, functional characteristics, and the application in food, vaccines, animal husbandry, and veterinary medicine. We summarized the features and improvements and then predicted the development prospects of NICE system.

Key words:Lactococcus lactis;host bacteria;nisin-controlled expression system (NICE);application