空肠弯曲菌CRISPR/Cas系统的研究进展

Research progress of CRISPR/Cas system in Campylobacter jejuni

摘要:

多数细菌都存在发挥免疫防御机制作用的CRISPR/Cas系统，在不同种属间呈现多态性。空肠弯曲菌是全球范围内重要的食源性病原菌，所致疾病也是典型的自限性疾病，其复杂的致病机制并未得到明确的解析，而空肠弯曲菌CRISPR/Cas系统的结构呈现多态性，研究两者关系仍存在诸多限制。本文从CRISPR/Cas系统在空肠弯曲菌中的结构、机制及技术应用等方面的研究进展进行综述，为探索空肠弯曲菌致病机制提供新思路。

关键词:空肠弯曲菌;CRISPR/Cas系统;结构;免疫防御

Abstract:

CRISPR/Cas system is distributed in most bacteria, with immune defense mechanisms and showing polymorphisms among different species. Campylobacter jejuni is an important foodborne pathogen worldwide, and the disease caused by infection with it is also a typical self-limiting disease. Its pathogenic mechanism is complex, which has not been clearly clarified, and together with the polymorphisms of CRISPR/Cas system in C.jejuni, there exist many limitations in analyzing the relationship between CRISPR/Cas system and C.jejuni. In this paper, the structure, mechanism and technical application of CRISPR/Cas system in C.jejuni were reviewed, which provided new ideas for exploring the pathogenic mechanism of C.jejuni.

Key words:Campylobacter jejuni;CRISPR/Cas system;structure;immune defense