一株红树林曲霉菌Aspergillus sp.WHUF0343的次级代谢产物研究

Secondary metabolites from a mangrove-derived fungus Aspergillus sp. WHUF0343

摘要:

【目的】研究分离自三亚亚龙湾红树林根系土壤的真菌Aspergillus sp.WHUF0343的次级代谢产物及其生物活性。【方法】利用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱等技术对该菌的发酵产物进行分离纯化；综合利用核磁共振波谱(NMR)和质谱(MS)等现代波谱技术以及与文献数据对照确定化合物的结构；分别采用肉汤微量稀释检测法和MTS法对化合物进行抗菌和肿瘤细胞毒活性测试。【结果】从真菌Aspergillus sp.WHUF0343的发酵产物共分离并鉴定了10个化合物，分别为isoechinulin A (1)、neoechinulin A (2)、neoechinulin E (3)、preechinulin (4)、neoechinulin D (5)、variecolorin J (6)、dehydroechinulin (7)、questinol (8)、emodin (9)和catenarin (10)。活性评价显示化合物2、9和10对幽门螺杆菌(Helicobacter pylori)和金黄色葡萄球菌(Staphylococcus aureus)及其耐药菌株具有较强的抑制活性；化合物1对3株肿瘤细胞B16、HepG2和MCF-7均具有细胞毒活性。【结论】Aspergillus sp.WHUF0343具有开发为微生物药物的潜在研究价值。

Abstract:

[Objective] To investigate secondary metabolites of the mangrove-derived fungus Aspergillus sp. WHUF0343 from the root soil of an unidentified mangrove plant sample collected in Yalong Bay, Sanya, China, and their activity. [Methods] The column chromatography on silica gel and Sephadex LH-20, and semi-preparative high-performance liquid chromatography (HPLC) were used to isolate and purify the secondary metabolites from the fermented extract of Aspergillus sp. WHUF0343. Their structures were elucidated by modern spectroscopy methods such as nuclear magnetic resonance (NMR) and mass spectrometry (MS) combined with relevant literature data. Their antibacterial and cytotoxic activities were evaluated by broth microdilution assay and MTS assay, respectively. [Results] Ten compounds were isolated, which were respectively identified as isoechinulin A (1), neoechinulin A (2), neoechinulin E (3), preechinulin (4), neoechinulin D (5), variecolorin J (6), dehydroechinulin (7), questinol (8), emodin (9) and catenarin (10). Compounds 2, 9 and 10 exhibited moderate inhibitory activities against Helicobacter pylori, Staphylococcus aureus and their drug-resistant strains. Compound 1 demonstrated cytotoxic activities against malignant melanoma cell line B16, human hepatocellular carcinoma cell line HepG2 and human breast cancer cell MCF-7. [Conclusion] Aspergillus sp. WHUF0343 has potential research value in microbial drugs.