噬菌体治疗小鼠肺部铜绿假单胞菌感染的研究

Phage therapy for the mouse pulmonary infection caused by Pseudomonas aeruginosa

摘要:

【目的】通过两种给药方式观察噬菌体鸡尾酒制剂对铜绿假单胞菌(Pseudomonas aeruginosa)肺部感染小鼠的疗效。【方法】将小鼠行气管切开术，经气管灌注2×10⁸ CFU/mL的铜绿假单胞菌悬液50 μL，0.5 h后分别给予噬菌体鸡尾酒滴鼻和腹腔注射，同时建立PBS滴鼻和腹腔注射对照组。待感染24 h后，观察小鼠的生理状态，并行细菌学、病理学与炎症因子水平等检查。【结果】噬菌体鸡尾酒制剂的治疗组小鼠，肺内的铜绿假单胞菌被清除；病理结果显示，治疗组小鼠肺部组织结构较完好、炎症程度较轻。【结论】该铜绿假单胞菌噬菌体鸡尾酒制剂在小鼠体内具有很强的抗菌作用，对肺部细菌感染具有一定的治疗效果。

Abstract:

[Objective]A murine pneumonia model infected by Pseudomonas aeruginosa was established and treated with a phage cocktail in two ways,and then the therapeutic effect was evaluated.[Methods] We injected 50 μL P.aeruginosa suspension of 2×10⁸ CFU/mL into the trachea after performing the tracheostomy in mice.After half an hour,a phage cocktail preparation was administrated into the infected mice through intranasal and intraperitoneal routes.At the same time,we established two control groups with phosphate-buffered saline in parallel.We then observed the physiological state of the mice and examined the bacteriological,pathological,and inflammatory levels 24 h post infection.[Results]P.aeruginosa was eliminated in the mice administrated with the phage cocktail preparation.The mice treated the phage cocktail showed intact lung tissue structure and slight inflammation compared with the control group.[Conclusion]The phage cocktail preparation can inhibit P.aeruginosa in mice and has a certain therapeutic effect on bacterial infections in the lungs.