不同地区的东亚飞蝗肠道微生物多样性分析

Diversity of gut microbiota in Locusta migratoria manilensis Meyen from different regions of China

摘要:

【目的】鉴于肠道微生物在昆虫环境适应过程中发挥重要作用，探索中国不同地区分布的东亚飞蝗肠道微生物多样性情况。【方法】本文利用Illumina NovaSeq测序平台对中国4个代表性蝗区的东亚飞蝗(Locusta migratoria manilensis Meyen)肠道微生物进行16S rRNA基因测序和分析，探究其多样性。【结果】分布区域、发育时期和性别对东亚飞蝗肠道微生物结构均影响明显，其中分布区域的影响最为显著，其次为性别和发育时期。多样性分析发现，采自广东省清远市和河北省沧州市的东亚飞蝗肠道微生物多样性差异性较大，所选取的8个环境因子中平均气温和降水量可影响东亚飞蝗的肠道菌群多样性。【结论】研究结果从共生微生物和环境因素角度解析了东亚飞蝗的环境适应性，为研制地理溯源技术和开发微生物制剂提供了科学依据。

Abstract:

[Objective] Gut microbiota plays an important role in the environmental adaptation of insects. This study aims to explore the diversity of gut microbiota in Locusta migratoria manilensis Meyen from different regions of China. [Methods] The Illumina NovaSeq platform was used to sequence the 16S rRna gene of gut microbiota in L. migratoria manilensis Meyen from four representative locust regions in China. [Results] Geographical location, development stage, and gender affected the structure of gut microbiota, among which geographical location had the most significant effect, followed by gender and development stage. The L. migratoria manilensis Meyen from Qingyuan City, Guangdong Province and Cangzhou City, Hebei Province showed the largest difference in the diversity of gut microbiota. Mean temperature and precipitation among the eight selected environmental factors may influence the gut microbiota diversity. [Conclusion] This study analyzed the environmental adaptability of L. migratoria manilensis Meyen from the perspective of symbiotic microorganisms and environmental factors, which provided a basis for the development of technology for tracing geographical origin and microbial preparations.