Abstract:Epigenetic control plays important roles in regulation of eukaryotic gene expression, which includes DNA methylation, histone modification, and chromatin remodeling. Among them, chromatin remodelers function by affecting the binding of histone-modifying enzymes and transcription factors. The chromatin remodeling complex INO80 is evolutionarily conserved and it promotes nucleosome sliding and eviction by using the energy obtained from ATP hydrolysis. In addition to the role in DNA replication and repair, INO80 regulates the gene expression in budding yeast Saccharomyces cerevisiae by altering DNA accessibility. This review summarized the classification and composition of chromatin remodeling complexes in S. cerevisiae and introduced the roles (including expulsion of RNA polymerase Ⅱ, response to signaling pathways, and alteration of gene expression levels) of the multi-subunit complex INO80 in the dynamic regulation of gene expression. Furthermore, the research progress in the role of INO80 in the response of S. cerevisiae to environmental stress was reviewed. Studying the functions of INO80 benefits understanding the delicate metabolic regulation mechanisms in eukaryotes, which provides a basis for developing microbial metabolic engineering and synthetic biology tools employing chromatin remodelers and other epigenetic regulators to improve stress tolerance and fermentation efficiency.
