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微生物学报

三株真菌对重金属锰的耐受性和吸附特性研究
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国家重点研发计划(2018YFD0200407)


Tolerance of three fungal species to heavy metal manganese and the adsorption of the metal
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    摘要:

    【目的】研究3种真菌对锰离子的耐受性,并研究其对溶液中Mn2+吸附的最佳条件和吸附机理,为治理锰离子污染提供技术参考。【方法】测定哈茨木霉(Trichoderma harzianum)、深绿木霉(Trichoderma atroviride)和棘孢木霉(Trichoderma asperellum)三株真菌的最低抑菌浓度(minimum inhibitory concentration,MIC),探究最佳吸附条件,并利用扫描电子显微镜(scanning electron microscopy-energy dispersive X-ray spectroscopy,SEM-EDS)和傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)对吸附前后菌体进行分析。【结果】哈茨木霉、深绿木霉、棘孢木霉对重金属锰耐受的浓度可达到1 600、1 800、2 000 mg/L,最佳吸附条件为pH为7,吸附时间80 h,温度28℃,吸附率最高可达23.7%,哈茨木霉参与吸附的官能团有-OH、胺基中的-C-N-、-C=O。棘孢木霉参与吸附的官能团有-OH和-NH。深绿木霉参与吸附的官能团主要有-C-H、磷酸基团P=O、P-OH和PO43-。【结论】哈茨木霉、深绿木霉、棘孢木霉对重金属锰有较好的吸附作用,可为治理土壤中的锰污染提供重要的参考和依据。

    Abstract:

    [Objective] To explore the tolerance of three fungal species to manganese ion, the optimal conditions for them to adsorb Mn2+ in solution, and the mechanism for the adsorption, and thus to provide technical reference for the control of manganese ion pollution.[Methods] The minimum inhibitory concentration (MIC) of Trichoderma harzianum, T. atroviride and T. asperellum was determined and the optimal adsorption conditions were explored. Based on scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR), fungal cells before and after the adsorption were analyzed. [Results] T. harzianum, T. atroviride, and T. asperellum could tolerate the maximum manganese concentration of 1 600 mg/L, 1 800 mg/L, and 2 000 mg/L, respectively. The optimum adsorption conditions are pH 7, adsorption time of 80 h, and temperature of 28℃, and the highest adsorption rate was up to 23.7%. The functional groups involved in the adsorption of T. harzianum were -OH, and -C-N- and-C=O in the amine group, and those of T. asperellum were -OH and -NH. The functional groups of T. atroviride were -C-H, and phosphate groups P=O, P-OH, and PO43-.[Conclusion] The screened T. harzianum, T. atroviride, and T. asperellum showed strong adsorption of heavy metal manganese, which can serve as a reference the treatment of manganese pollution in soil.

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韦天慧,宋金柱,刘诗宇,宫殿良,王棋,孙瑶. 三株真菌对重金属锰的耐受性和吸附特性研究. 微生物学报, 2023, 63(3): 1023-1034

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历史
  • 收稿日期:2022-07-08
  • 最后修改日期:
  • 录用日期:2022-09-05
  • 在线发布日期: 2023-03-08
  • 出版日期: 2023-03-04