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地衣芽孢杆菌对脂多糖诱导的仔猪肝脏炎症及抗氧化功能的影响
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浙江省重点研发计划(2021C02008);浙江省“尖兵”“领雁”研发攻关计划(2022C02043,2022C02059);杭州市农业和社会发展一般项目(202203B18);浙江省重点农业企业研究院(2021Y30004)


Effects of Bacillus licheniformis on lipopolysaccharide-induced liver inflammation and antioxidant function in weaned piglets
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    摘要:

    【目的】本文旨在研究地衣芽孢杆菌(Bacillus licheniformis HJ0135)对脂多糖(lipopolysaccharide,LPS)应激断奶仔猪肝脏指数、炎症因子、抗氧化功能及相关基因表达的影响。【方法】选择24头28日龄健康状况良好的三元杂交断奶仔猪(杜×长×大),随机分成4个试验组:对照组(control,CON),500 mg/kg地衣芽孢杆菌HJ0135组(BL)、LPS应激组(LPS)以及LPS+500 mg/kg地衣芽孢杆菌HJ0135组(BL-LPS),在试验第28天,给LPS组和BL-LPS组仔猪腹腔注射1.5 mL浓度为1 mg/mL的LPS,给CON组和BL组仔猪注射等量生理盐水,1.5 h后屠宰并采样。【结果】与CON组相比,BL组血清谷草转氨酶(aspartate aminotransferase,AST)和谷丙转氨酶(alanine aminotransferase,ALT)的含量降低,肝脏的总抗氧化能力(total antioxidant capacity,T-AOC)提升且过氧化氢酶(catalase,CAT)和总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性均增强。肝脏中促炎因子白细胞介素-6(interleukin-6,IL-6)含量减少,同时肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)含量下降,肝脏CATSOD1SOD2、谷胱甘肽过氧化物酶-1(glutathione peroxidase,GSH-Px1)、核因子E2相关因子(nuclear factor erythroid 2-related factor 2,Nrf2)、醌氧化还原酶1(NADPH:quinine oxidoreductase 1,NQO1)、Toll样受体4(Toll-like receptors 4,TLR4)、髓样分化因子(myeloid differentiation factor 88,MyD88)和核因子-κB (nuclear factor kappa beta,NF-κB)的基因表达水平增高;与LPS组相比,BL-LPS组肝脏指数降低,血清AST和ALT的含量降低,肝脏中CAT、GSH-Px、T-AOC含量均提高,而丙二醛(malondialdehyde,MDA)含量降低。另外,肝脏炎症因子白细胞介素-1β(interleukin-1β,IL-1β)、IL-6和TNF-α含量减少,而白细胞介素-10(interleukin-10,IL-10)的含量增加,肝脏CATSOD2GSH-Px1Nrf2TLR4MyD88NF-κB的基因表达量提高。【结论】断奶仔猪日粮中添加地衣芽孢杆菌HJ0135,能够激活Nrf2和TLR4相关基因表达,降低脂多糖刺激引起的肝脏损伤和炎症因子的分泌,提高断奶仔猪抗氧化能力。

    Abstract:

    [Objective] To investigate the protective effects of Bacillus licheniformis HJ0135 on the liver index, inflammatory cytokines, antioxidant function, and related gene expression of the weaned piglets exposed to lipopolysaccharide (LPS). [Methods] A total of 24 healthy 28-day-old weaned piglets (Duroc×Landrace×Yorkshire) were randomly assigned into 4 groups:control (CON) group, 500 mg/kg B. licheniformis HJ0135 (BL) group, LPS stress (LPS) group, and LPS+500 mg/kg B. licheniformis HJ0135 (BL-LPS) group, with 6 piglets in each group. On day 28 of the experiment, the piglets in the LPS and BL-LPS groups were injected intraperitoneally with 1.5 mL of 1 mg/mL LPS, and those in the CON and BL groups with equal amounts of saline. All the piglets were sacrificed after 1.5 h. [Results] Compared with the CON group, BL treatment decreased the content of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), increased the total antioxidant capacity (T-AOC), the activities of catalase (CAT) and total superoxide dismutase (T-SOD), reduced the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the liver, and up-regulated the expression of CAT, superoxide dismutase 1 (SOD1), SOD2, glutathione peroxidase 1 (GSH-Px1), nuclear factor erythroid 2-related factor (Nrf2), NADPH:quinone oxidoreductase 1 (NQO1), Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor-𝜅B (NF-𝜅B) in the liver. Compared with the LPS group, BL-LPS treatment decreased liver index and serum AST and ALT levels, increased the levels of CAT, GSH-Px, and T-AOC, and reduced the content of malondialdehyde (MDA), interleukin-1β (IL-1β), IL-6, and TNF-α. In addition, BL-LPS treatment up-regulated the mRNA levels of CAT, SOD2, GSH-Px1, Nrf2, TLR4, MyD88, and NF-𝜅B in the liver. [Conclusion] Dietary B. licheniformis HJ0135 can activate the expression of Nrf2 and TLR4-related genes, reduce liver damage and inflammatory cytokine secretion induced by lipopolysaccharide, and improve the antioxidant capacity in weaned piglets.

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俞晓蓉,代兵,刘金松,崔振川,史雪艳,吴艳萍,张瑞强,杨彩梅. 地衣芽孢杆菌对脂多糖诱导的仔猪肝脏炎症及抗氧化功能的影响. 微生物学报, 2023, 63(3): 1283-1294

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  • 收稿日期:2022-08-03
  • 最后修改日期:
  • 录用日期:2022-10-30
  • 在线发布日期: 2023-03-08
  • 出版日期: 2023-03-04