古菌RecJ蛋白的研究进展
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省大学生创新创业训练计划(202211117084Y);江苏省自然科学基金(BK20191219)


Research progress of archaeal RecJ protein
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    RecJ蛋白属于aspartate-histidine-histidine (DHH)磷酸酯酶超家族,存在于细菌、真核生物和古菌中。细菌RecJ蛋白是一种5′→3′ ssDNA外切酶,参与错配修复、同源重组、碱基切除修复等生物学过程。真核生物cell division cycle 45 (Cdc45)蛋白是细菌RecJ核酸酶的同源物,但不具有核酸酶活性。Cdc45蛋白能够与minichromosome maintenance (MCM)和Go-Ichi-Ni-San (GINS)形成Cdc45-MCM-GINS (CMG)复合物,是真核生物DNA复制的重要组分。在古菌中,几乎所有基因组已测序的古菌均编码一种或多种RecJ蛋白同源物。与细菌RecJ核酸酶不同,古菌RecJ蛋白具有多样化的核酸酶活性,并且能够与MCM和GINS形成类似于真核生物CMG的复合物。因此,古菌RecJ蛋白是参与古菌DNA复制、修复和重组的重要成分。基于目前古菌RecJ蛋白的研究报道,本文综述了古菌RecJ蛋白的活性、结构与功能方面的研究进展,聚焦于不同古菌RecJ蛋白以及它们与细菌RecJ核酸酶和真核生物RecJ同源物的异同点,并对古菌RecJ蛋白未来的研究方向进行了展望。

    Abstract:

    RecJ proteins, the members of aspartate-histidine-histidine (DHH) superfamily of phosphoesterases, are ubiquitous in bacteria, eukaryotes, and archaea. In bacteria, the archetypal RecJ protein is a 5′→3′ ssDNA exonuclease that functions in biological processes such as mismatch repair, homologous recombination, and base excision repair. Cell division cycle 45 (Cdc45) protein, a homologue of bacterial RecJ nuclease, is essential in eukaryotes, but has no nuclease activity. Cdc45 protein forms Cdc45-MCM-GINS (CMG) complex with minichromosome maintenance (MCM) and Go-Ichi-Ni-Sa (GINS), and the complex plays a key role in DNA replication in eukaryotes. Almost all archaea whose genomes have been sequenced encode one or more RecJ protein homologues. Unlike bacterial RecJ nuclease, archaeal RecJ protein has diverse nuclease activities and can form a complex with MCM and GINS which shows functions similar to eukaryotic CMG complex. Thus, archaeal RecJ protein is an important component that is involved in archaeal DNA replication, repair and recombination. Based on current reports on archaeal RecJ protein, this study reviewed the activities, structures, and functions of archaeal RecJ protein, focusing on similarities and differences between different archaeal RecJ proteins and between archaeal RecJ proteins and bacterial RecJ nucleases/eukaryotic RecJ homologues. In addition, we summarized the research trends in archaeal RecJ protein.

    参考文献
    相似文献
    引证文献
引用本文

施静茹,张立奎. 古菌RecJ蛋白的研究进展. 微生物学报, 2023, 63(4): 1318-1328

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-08-11
  • 最后修改日期:2022-10-14
  • 录用日期:
  • 在线发布日期: 2023-04-06
  • 出版日期:
文章二维码