红色红曲菌M7的丝衣霉酸基因簇及其功能研究

Study on the byssochlamic acid gene cluster of Monascus ruber M7 and its function

摘要:

【目的】明确红色红曲菌(Monascus ruber) M7的基因组中是否存在丝衣霉酸(byssochlamic acid, BA)基因簇，并探讨BA基因簇中聚酮合酶基因mr-Bys及3-羟酰基辅酶A脱氢酶基因mr-hdh对BA产生的影响。【方法】采用生物信息学方法对M7基因组进行分析，以确定BA候选基因簇；以基因敲除及过表达方法研究mr-Bys和mr-hdh对BA产生的影响；以超高效液相色谱(ultra-performance liquid chromatography, UPLC)和质谱(mass spectrometry, MS)方法分析BA。【结果】在红色红曲菌M7基因组中发现了BA基因簇，敲除mr-Bys后，BA消失，而敲除和过表达mr-hdh均可影响BA的产生。【结论】红色红曲菌M7基因组中存在BA基因簇，可产生BA。研究结果不仅丰富了红曲菌次生代谢产物及其合成途径的相关研究，也为以红曲菌为菌种开发新产品奠定了基础。

Abstract:

[Objective] To determine whether the byssochlamic acid (BA) gene cluster exists in the genome of Monascus ruber M7, and to explore the effect of polyketide synthase gene mr-Bys and 3-hydroxyacyl-CoA dehydrogenase gene mr-hdh in BA gene cluster on BA production. [Methods] The M7 genome was analyzed by bioinformatics methods to identify the BA candidate gene clusters. The effects of mr-Bys and mr-hdh on BA production were studied by the gene knockout and over-expression methods. BA was analyzed by ultra-performance liquid chromatography (UPLC) and mass spectrometry (MS). [Results] A BA gene cluster was found in the genome of M. ruber M7. After knocking out mr-Bys, BA disappeared, while both knockout and over-expression of mr-hdh affected BA production by M. ruber M7. [Conclusion] There is a BA gene cluster in the genome of M. ruber M7, which can produce BA. The research results enrich the research on the secondary metabolites of Monascus spp. and their synthetic pathways and lay a foundation for the development of new products using Monascus spp.