利福平对红裸须摇蚊幼虫肠道微生物群落结构及功能的潜在影响

Potential effects of rifampin on the structure and the function of gut microbiota in Propsilocerus akamusi larvae

摘要:

【目的】摇蚊是水生生态系统中重要的昆虫种类之一，其肠道微生物与个体生长发育、环境适应等过程密切相关，本研究旨在探究抗生素处理对摇蚊幼虫肠道微生物群落结构及功能的潜在影响。【方法】利用16S rRNA基因扩增子测序技术对利福平处理的红裸须摇蚊(Propsilocerus akamusi)幼虫肠道内容物中的菌群进行分析和比较，应用Tax4Fun法对其肠道菌群功能进行预测。【结果】利福平处理能够改变红裸须摇蚊幼虫肠道群落结构和多样性，宿主肠道菌群中拟杆菌门(Bacteroidota) (P<0.05)以及脱铁杆菌门(Deferribacterota) (P<0.001)的相对丰度显著上升，而变形菌门(Proteobacteria)与厚壁菌门(Firmicutes)相对丰度有所下降。在属水平上，利福平处理使耶尔森菌属(Yersinia)、假单胞菌属(Pseudomonas)、脱硫弧菌属(Desulfovibrio) 的相对丰度有所降低，其中脱硫弧菌属(Desulfovibrio)显著降低。与此同时，共线性网络分析表明利福平处理后细菌群落稳定性大幅下降，菌种之间关联性显著减弱。通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)通路注释预测出红裸须摇蚊幼虫肠道菌群基因与基因信息处理、新陈代谢、人类疾病等功能相关，利福平处理可以使肠道菌群基因的抗药性功能显著上升，而内分泌和代谢疾病功能显著下降。【结论】研究结果揭示了抗生素利福平对红裸须摇蚊幼虫肠道细菌群落结构及功能的潜在影响，为进一步探索摇蚊肠道菌群发挥的必要作用奠定理论基础。

Abstract:

[Objective] Propsilocerus akamusi is one of the most important insects in aquatic ecosystem, and its gut microbiota is closely related to individual growth and development, environmental adaptation, and other physiological processes. This study aimed to investigate the potential effects of antibiotic treatment on the structure and the function of gut microbiota in P. akamusi larvae. [Methods] The microbes in the gut content of P. akamusi larvae treated by rifampin were analyzed and compared by using the 16S rRNA amplicon sequencing, and the functional prediction of gut microbial genes was conducted by using Tax4Fun method. [Results] Rifampin altered the structure and the diversity of gut microbiota in P. akamusi larvae. After rifampin treatment, the relative abundance of the bacterial phylum Bacteroidota (P<0.05) and Deferribacterota (P<0.001) was up-regulated, while that of Proteobacteria and Firmicutes was down-regulated. At the genus level, treatment with rifampin resulted in a significant decrease in the relative abundance of Yersinia,Pseudomonas, and Desulfovibrio (P<0.05). At the same time, the co-occurrence network showed that the stability of the microbiota coupled with the correlation between bacterial species were significantly weakened after rifampin treatment. Additionally, the Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis predicted that genes in the gut microbiota of P. akamusi larvae were mostly involved in the genetic information processing, metabolism, and human disease. Rifampin significantly increased the expression level of genes involved in drug resistance, while decreased those participating in endocrine and metabolic disease function. [Conclusion] The above-mentioned results reveal the potential effects of rifampin on the structure and the function of the gut microbiota in P. akamusi larvae, which build a fundamental basis to further explore the essential roles of the gut microbiota in P. akamusi.