以N-甲基吡咯烷酮(NMP)为电子供体进行反硝化的可行性及实验研究
作者:
基金项目:

上海市地方高校能力建设项目(201607050300)


Feasibility of N-methylpyrrolidone (NMP) as an electron donor for denitrification
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [25]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    【目的】利用N-甲基吡咯烷酮(N-methylpyrrolidone, NMP)作为电子供体进行反硝化实验,以实现废水资源化。【方法】分别将NMP废水和葡萄糖作为电子供体加入到模拟的城市污水处理尾水中进行反硝化,比较2种电子供体去除硝酸盐的规律。同时考查NMP在反硝化过程中的氮素释放规律,并对所释放的氮素进行后续处理。最后再对它们作为电子供体时的反硝化污泥采用高通量测序,从微生物群落的角度分析NMP作为电子供体时其作用机理是否相同。【结果】当以NMP为电子供体时,硝酸盐氮的去除速率比葡萄糖为电子供体时要快67%。在8 h的反硝化结束后,剩余的硝酸盐氮、累积的亚硝酸盐氮和NMP本身所释放氨氮之和的总氮,与葡萄糖为电子供体时相近。【结论】NMP废水可以作为电子供体用于城镇污水处理厂的深度脱氮。对2种碳源所驯化的反硝化污泥样品进行高通量分析表明,NMP与葡萄糖作为电子供体用于反硝化反应时,相关的作用机理是不同的。该项研究结果对利用含氮杂环化合物作为电子供体进行反硝化具有重要的理论指导意义。

    Abstract:

    [Objective] N-methylpyrrolidone (NMP) was used as an electron donor for denitrification to treat wastewater. [Methods] NMP and glucose as electron donors were respectively added into the simulated effluent from wastewater treatment plant for denitrification, during which they were compared for the removal of nitrates. The fate of nitrogen released during the denitrification was studied, and the released nitrogen was further treated. Furthermore, we submitted the sludge samples acclimated with NMP and glucose for high-throughput sequencing to investigate their mechanisms of denitrification. [Results] The NMP-acclimating sludge showed the nitrate-N removal rate 67% higher than the glucose-acclimating sludge. After 8 h of denitrification with NMP as the electron donor, the total nitrogen (TN) composed of residual nitrate N, generated nitrite N, and released ammonium N from NMP biodegradation was similar to the TN after the denitrification with glucose as the electron donor. [Conclusion] The wastewater containing NMP could be used as the electron donor for deep nitrate removal, although it was directly treated as the wastewater before. High-throughput sequencing indicated that NMP was different from glucose in the mechanism of denitrification when being used as the electron donor. As one of the nitrogen-containing heterocyclic compounds, NMP releases the electron donor for denitrification through the biodegradation of organic acids after the cleavage of NMP ring. However, glucose releases its electron donor for denitrification through the biodegradation without ring cleavage because of its simple structure. The results have theoretical significance for utilizing nitrogen-containing heterocyclic compounds as electron donors for denitrification.

    参考文献
    [1] 刘梦, 伯鑫, 孟凡琳, 金正宇, 王龙飞, 于浪, 赵晓宏. 2015年中国城镇污水处理厂达标排放评估[J]. 环境工程, 2017, 35(10): 77-81, 90. LIU M, BO X, MENG FL, JIN ZY, WANG LF, YU L, ZHAO XH. Standard discharge evaluation of Chinese urbansewage plants in 2015 based on continuous emission monitoring system data[J]. Environmental Engineering, 2017, 35(10): 77-81, 90 (in Chinese).
    [2] 鲁杨川, 张城铭.我国城镇污水中污染物排放现状解析及展望[J]. 四川环境, 2022, 41(3): 273-278. LU YC, ZHANG CM. Analysis and prospect of pollutant emissions in urban sewage in China[J]. Sichuan Environment, 2022, 41(3): 273-278 (in Chinese).
    [3] 何星海, 马世豪, 罗孜. 北京市《城镇污水处理厂水污染物排放标准》解读[J].给水排水, 2013, 49(10): 123-127. HE XH, MA SH, LUO Z. Explanation of Beijing local standard of discharge standard of water pollutants for municipal wastewater treatment plants[J]. Water & Wastewater Engineering, 2013, 49(10): 123-127 (in Chinese).
    [4] LU QY, ZHOU JQ, ZHU G, TAN C, CHEN SY, ZHU XH, YAN N, ZHANG YM, XU QE, PAN BF, RITTMAN NBE. Anoxic/oxic treatment without biomass recycle[J]. The Science of the Total Environment, 2022, 834: 155166.
    [5] 余琴芳, 章诗璐, 杜敬, 刘佳. 反硝化生物滤池碳源选择与运行方式优化研究[J]. 水处理技术, 2022, 48(9): 113-117, 122. YU QF, ZHANG SL, DU J, LIU J. Study on the carbon source selection and operation optimization of denitrification biofilter[J]. Technology of Water Treatment, 2022, 48(9): 113-117, 122 (in Chinese).
    [6] 李宛谊. 啤酒废水资源化研究进展[J]. 中国资源综合利用, 2021, 39(10): 141-144, 147. LI WY. Research progress of brewery wastewater resource utilization[J]. China Resources Comprehensive Utilization, 2021, 39(10): 141-144, 147 (in Chinese).
    [7] 谢甜. 基于啤酒废水为碳源的短程反硝化及其与厌氧氨氧化耦合脱氮研究[D]. 青岛: 青岛科技大学硕士学位论文, 2022. XIE T. Partial denitrification based on brewery wastewater as carbon source and nitrogen removal via combiningpartial denitrification with anammox[D]. Qingdao: Master’s Thesis of Qingdao University of Science & Technology, 2022 (in Chinese).
    [8] 吴正岭. γ-丁内酯与N-甲基吡咯烷酮联产装置废水处理研究[J]. 精细与专用化学品, 2015, 23(6): 44-47. WU ZL. Study on the wastewater treatment in the cogeneration device with the N-methylpyrrolidone and gamma-butyrolacton[J]. Fine and Specialty Chemicals, 2015, 23(6): 44-47 (in Chinese).
    [9] 陈松筠, 诸晓慧, 梁斌, 曲征野, 项彩鑫, 张永明. 以N-甲基吡咯烷酮为电子供体的反硝化[J]. 上海师范大学学报(自然科学版), 2022, 51(4): 492-498. CHEN SJ, ZHU XH, LIANG B, QU ZY, XIANG CX, ZHANG YM. N-methylpyrrolidone used as electron donor for denitrification[J]. Journal of Shanghai Normal University (Natural Sciences Edition), 2022, 51(4): 492-498 (in Chinese).
    [10] WANG J, LIU X, JIANG X, ZHANG L, HOU C, SU G, WANG L, MU Y, SHEN J. Nitrate stimulation of N-methylpyrrolidone biodegradation by Paracoccuspantotrophus: metabolite mechanism and genomic characterization[J]. Bioresource Technology, 2019, 294: 122185.
    [11] ZHU G, ZHANG YM, CHEN SY, WANG L, ZHANG ZC, RITTMANN BE. How bioaugmentation with Comamonastestosteroni accelerates pyridine mono-oxygenation and mineralization[J]. Environmental Research, 2021, 193: 110553.
    [12] QUAST C, PRUESSE E, YILMAZ P, GERKEN J, SCHWEER T, YARZA P, PEPLIES J, GLÖCKNERFO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools[J]. Nucleic Acids Research, 2013, 41(D1): D590-D596.
    [13] CAI S, CAI T, LIU S, YANG Q, HE J, CHEN L, HU J. Biodegradation of N-methylpyrrolidone by Paracoccus sp. NMD-4 and its degradation pathway[J]. International Biodeterioration & Biodegradation, 2014, 93: 70-77.
    [14] 诸晓慧. N-甲基-2-吡咯烷酮的生物降解与利用[D]. 上海: 上海师范大学硕士学位论文, 2022. ZHU XH. Biodegradation and utilization of N- methyl-2-pyrrolidone[D]. Shanghai: Master’s Thesis of Shanghai Normal University, 2022 (in Chinese).
    [15] CARNERUP M, ÅKESSON AB, GJÖNSSON BA. Determination of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in human plasma and urine using liquid chromatography-electrospray tandem mass spectrometry[J]. Journal of Chromatography B: Biomedical Sciences and Applications, 2001, 761(1): 107-113.
    [16] WANG T, LIN Z, KUANG B, NI Z, CHEN X, GUO B, ZHU G, BAI S. Electroactive algae-bacteria wetlands for the treatment of micro-polluted aquaculture wastewater: pilot-scale verification[J]. Biochemical Engineering Journal, 2022, 184: 108471.
    [17] KIM E, YULISA A, KIM S, HWANG S. Monitoring microbial community structure and variations in a full-scale petroleum refinery wastewater treatment plant[J]. Bioresource Technology, 2020, 306: 123178.
    [18] LI JY, DU QP, PENG HQ, ZHANG YL, BI YQ, SHI Y, XU YB, LIU T. Optimization of biochemical oxygen demand to total nitrogen ratio for treating landfill leachate in a single-stage partial nitrification-denitrification system[J]. Journal of Cleaner Production, 2020, 266: 121809.
    [19] LIU T, HE XL, JIA GY, XU JW, QUAN X, YOU SJ. Simultaneous nitrification and denitrification process using novel surface-modified suspended carriers for the treatment of real domestic wastewater[J]. Chemosphere, 2020, 247: 125831.
    [20] ZHANG YM, CHANG L, YAN N, TANG YX, LIU R, RITTMANN BE. UV photolysis for accelerating pyridine biodegradation[J]. Environmental Science & Technology, 2014, 48(1): 649-655.
    [21] TANG YX, ZHANG YM, YAN N, LIU R, ITTMANN BE. The role of electron donors generated from UV photolysis for accelerating pyridine biodegradation[J]. Biotechnology and Bioengineering, 2015, 112(9): 1792-1800.
    [22] LIU YX, ZHANG Q, LV YK, REN RP. Pyridine degradation characteristics of a newly isolated bacterial strain and its application with a novel reactor for the further treatment in pyridine wastewater[J]. Process Biochemistry, 2020, 95: 64-70.
    [23] REN T, CHI YL, WANG Y, SHI X, JIN X, JIN PK. Diversified metabolism makes novel Thauera strain highly competitive in low carbon wastewater treatment[J]. Water Research, 2021, 206: 117742.
    [24] ZHANG LG, BAN QY, LI JZ, WAN CL. Functional bacterial and archaeal dynamics dictated by pH stress during sugar refinery wastewater in a UASB[J]. Bioresource Technology, 2019, 288: 121464.
    [25] ZHU WT, ZHANG XH, YUAN SC, SANG WJ, TANG XH, ZHANG SY. Effects of different aeration strategies on removal of organics, nitrogen and phosphorus in sequencing batch biofilm reactor (SBBR): performance, microbial community and nitrogen cycling pathways[J]. Journal of Water Process Engineering, 2022, 49: 103118.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

梁斌,谭学军,陈松筠,张永明. 以N-甲基吡咯烷酮(NMP)为电子供体进行反硝化的可行性及实验研究[J]. 微生物学报, 2023, 63(7): 2899-2908

复制
分享
文章指标
  • 点击次数:195
  • 下载次数: 829
  • HTML阅读次数: 985
  • 引用次数: 0
历史
  • 收稿日期:2022-11-16
  • 最后修改日期:2023-02-16
  • 在线发布日期: 2023-07-05
  • 出版日期: 2023-07-04
文章二维码