内生真菌印度梨形孢诱导小麦抗根腐病作用研究

Endophytic fungus Piriformospora indica induces wheats against root rot

摘要:

【目的】明确印度梨形孢(Piriformospora indica)诱导小麦对根腐病产生抗性的作用机制。【方法】用印度梨形孢悬液浸种，以无菌培养液为对照，用病原菌禾谷镰孢菌(Fusarium graminearum)侵染小麦，对其相关生理生化指标及转录组变化进行分析。【结果】禾谷镰孢菌能诱导小麦产生过氧化氢，降低细胞内水含量，破坏细胞膜的稳定性；根部定殖印度梨形孢的小麦细胞内抗氧化酶活性增强，活性氧自由基含量降低，胞内水含量提高，细胞膜稳定性增强；印度梨形孢定殖能改变由于病原菌引起的mRNA转录组变化，抗性相关基因的表达增强。综合表明印度梨形孢定殖能有效地提高小麦对禾谷镰孢菌的抗性。【结论】研究结果为深入理解植物与微生物互作、开发新型高效环保抗根腐病生物制剂提供理论和实验依据。

Abstract:

[Objective] To clarify the mechanism of Piriformospora indica in inducing wheats against root rot. [Methods] Seeds were soaked in the suspension of P. indica, and the medium without P. indica was used as control. After Fusarium graminearum infecting wheats, this study analyzed the physiological and biochemical indexes and transcriptome changes. [Results] The pathogen F. graminearum induced the production of hydrogen peroxide in wheats, reduced the intracellular water content, and destroyed the stability of the membrane. The colonization of P. indica increased the activity of intracellular antioxidant enzymes and reduced the accumulation of oxygen free radicals in cells. Intracellular water content was further maintained, and the stability of membranes was enhanced. The colonization of P. indica also reprogrammed the mRNA transcriptome changes caused by F. graminearum. The expression of resistance-related genes was enhanced. Based on the above results, P. indica effectively improved the resistance of wheats to F. graminearum. [Conclusion] The implementation of the work will provide theoretical basis and experimental basis for in-depth understanding of plant-microbe interaction and development of new efficient and environmentally friendly root rot agents.