双碱性氨基酸内肽酶末端剪切修饰显著提高酶活及其高效水解大豆蛋白

Terminal truncation improves the activity and soybean protein hydrolysis efficiency of dibasic endopeptidase

摘要:

【目的】 将酿酒酵母(Saccharomyces cerevisiae)和毕赤亚库德里亚夫泽维(Pichia kudriavzevii)来源的双碱性氨基酸内肽酶基因sckex2和pkkex2克隆到大肠杆菌(Escherichia coli) BL21中,实现双碱性氨基酸内肽酶的异源表达,研究重组酶的酶学性质及其与碱性蛋白酶的协同高效水解大豆蛋白释放出小分子活性肽的作用。【方法】 按照大肠杆菌的密码子偏好性,对S. cerevisiae和P. kudriavzevii的kex2基因进行优化,分析KEX2蛋白的非功能区域,对其C-末端、N-末端氨基酸进行剪切修饰获得4种突变酶基因sckex2∆3、sckex2∆4、pkkex2∆3和pkkex2∆4,构建在载体pGEX-6P-1上,转入E. coli BL21感受态细胞中,经DNA测序验证,获得重组菌株E. coli BL21/pGEX-ScKEX2∆3、E. coli BL21/pGEX-ScKEX2∆4、E. coli BL21/pGEX-PkKEX2∆3和E. coli BL21/pGEX-PkKEX2∆4。利用GST亲和层析柱和PreScission蛋白酶对重组酶进行分离纯化,研究纯酶pH和温度稳定性等酶学性质。以碱性蛋白酶单独水解大豆分离蛋白为对照,重组双碱性氨基酸内肽酶与碱性蛋白酶协同水解大豆蛋白,测定水解产物中小分子活性肽组分。【结果】 重组双碱性氨基酸内肽酶野生型和突变酶在E. coli BL21中可溶性表达,SDS-PAGE分析表明纯化的重组酶显示单一条带,在最适条件下,野生型酶几乎没有酶活,突变体最高比酶活达到47.32 U/g,K_m值为23.61μmol/L,k_cat值为50.18 s⁻¹,k_cat/K_m值为2 125.06 L/(mmol·s)。当重组酶在pH 5.0孵育2 d后,相对酶活力保留最高为40%以上。在35℃下孵育1 h后酶活力仍能保留60%以上。重组双碱性氨基酸内肽酶与碱性蛋白酶协同水解大豆分离蛋白,水解产物中超过39%为分子量小于500 Da的活性肽,且分子量小于100 Da的活性肽的含量比碱性蛋白酶单独水解时高50%。【结论】 通过末端剪切修饰,获得在大肠杆菌中可溶性表达的双碱性氨基酸内肽酶截短突变体,其重组酶催化效率高,能够高效水解大豆蛋白获得活性小分子肽,该研究为富含蛋白质生物资源的增值及高效水解奠定了坚实的研究基础。

关键词:双碱性氨基酸内肽酶;大肠杆菌;酶学性质;大豆蛋白;活性肽

Abstract:

[Objective] To realize the heterologous expression of dibasic endopeptidase genes sckex2 and pkkex2 from Saccharomyces cerevisiae and Pichia kudriavzevii in Escherichia coli BL21 and then study the properties of the recombinant KEX2 enzymes and their efficiency of hydrolyzing soybean protein in collaboration with alkaline protease. [Methods] The kex2 from S. cerevisiae and P. kudriavzevii was optimized according to the codon bias of E. coli. After analysis of non-functional region, we redesigned their C-terminal and N-terminal residues by truncation. Four truncated genes, sckex2∆3, sckex2∆4, pkkex2∆3, and pkkex2∆4, were respectively cloned into the vector pGEX-6P-1, and the recombinant plasmids were transformed into E. coli BL21 competent cells. After confirmed by DNA sequencing, the four recombinant strains, E. coli BL21/pGEX-ScKEX2∆3, E. coli BL21/pGEX-ScKEX2∆4, E. coli BL21/pGEX-PkKEX2∆3, and E. coli BL21/pGEX-PkKEX2∆4, were constructed. The recombinant enzymes were purified by GST affinity chromatography and PreScission protease and then their pH stability and thermal stability were determined. With the soybean protein hydrolysis by alkaline protease alone as the control, the yield of small-molecule peptides from the hydrolysis with the recombinant KEX2 in collaboration with alkaline protease was determined. [Results] The recombinant KEX2 and the wild type showed soluble expression in E. coli BL21. SDS-PAGE showed that the purified recombinant enzymes produced single bands. Under the optimal conditions, the wild type showed no enzyme activity, while the mutants showed the maximum specific activity of 47.32 U/g, K_m of 23.61 μmol/L, k_cat of 50.18 s⁻¹, and k_cat/K_m of 2 125.06 L/(mmol·s). After incubation at pH 5.0 for 2 d, the maximum relative activity of the recombinant enzymes was more than 40%. After incubation at 35℃ for 1 h, the relative activity was still over 60%. When the recombinant KEX2 and alkaline protease were used together to hydrolyze soybean protein, more than 39% of the hydrolysates were active peptides with molecular weight less than 500 Da, and the content of active peptides with the molecular weight less than 100 Da was 50% higher than that from the hydrolysis with the alkaline protease alone. [Conclusion] Through terminal truncation, the soluble expression of truncated KEX2 mutants was realized in E. coli. The recombinant enzymes demonstrate high catalytic efficiency and can efficiently hydrolyze soybean protein to release active peptides. This work lays a solid foundation for the value addition and deep hydrolysis of protein-rich biological resources.

Key words:dibasic endopeptidase (KEX2);Escherichia coli;enzymatic properties;soybean protein;active peptides