细菌中介导多重耐药的Tn7转座子研究进展

Research progress in Tn7 transposons mediating multidrug resistance in bacteria

摘要:

转座子是介导细菌耐药性传播的重要可移动遗传元件。Tn7转座子与细菌耐药密切相关，其携带转座模块和Ⅱ类整合子系统。Tn7编码转座相关蛋白TnsABCDE进行“剪切-粘贴”机制转座，转座核心TnsABC也可与三链DNA或Cas-RNA复合物结合实现转座。近年来新发现了多种介导多重耐药的Tn7转座子，其在介导细菌抗生素、消毒剂和重金属抗性基因的获得、传播扩散等方面发挥了重要作用。本文综述了细菌中Tn7转座子的遗传结构、转座机制、流行以及新发现的介导多重耐药的Tn7转座子，以期为细菌中Tn7转座子的深入研究提供参考。

Abstract:

Transposons are important mobile genetic elements that mediate the spread of antimicrobial resistance. The transposon Tn7 is closely associated with antimicrobial resistance, carrying a transposition module and a class II integron. Tn7 encodes the transposition-associated proteins TnsABCDE for cut-and-paste transposition, and the transposition core machinery TnsABC can bind to triple-stranded DNA or Cas (clustered regularly interspaced short palindromic repeats associated)-RNA complexes to achieve transposition. In recent years, researchers have identified several novel Tn7 transposons mediating multidrug resistance, which play a role in mediating the acquisition and spread of bacterial genes conferring resistance to antibiotics, disinfectants, and heavy metals. In this paper, we review the genetic structure, transposition mechanism, prevalence of Tn7 transposons and novel Tn7 transposons mediating multidrug resistance in bacteria, with a view to providing a reference for the in-depth study of Tn7 transposons in bacteria.