单增李斯特菌LPXTG蛋白Lmo0880在感染致病中的作用

Role of LPXTG-anchored protein Lmo0880 in Listeria monocytogenes infection

摘要:

【目的】通过构建单核细胞增生李斯特菌(单增李斯特菌) LPXTG蛋白Lmo0880的基因缺失菌株和回补菌株,探究Lmo0880在细菌生长、细胞感染和宿主感染等方面发挥的作用。【方法】利用同源重组原理构建lmo0880的基因缺失株及回补株,比较野生株、缺失株和回补株在生长能力、细胞黏附与侵袭和胞内增殖能力等方面的差异,从而鉴定Lmo0880在单增李斯特菌感染宿主中的作用。【结果】缺失lmo0880基因后,单增李斯特菌在生长能力上无明显变化;对细胞的黏附能力无显著差异,但对细胞侵袭能力、胞内增殖能力、小鼠致病力和小鼠组织定殖能力显著降低。【结论】本研究阐明了单增李斯特菌LPXTG蛋白Lmo0880在细胞侵袭、胞内增殖和组织定殖等方面发挥的重要作用。

Abstract:

【Objective】 To generate Listeria monocytogenes strains with lmo0880 deleted and complemented strains, so as to investigate the roles of Lmo0880 in bacterial infection in a host. 【Methods】 The lmo0880-deleted strain was generated by homologous recombination, and the complementary strain was constructed by introducing an integrative plasmid carrying lmo0880 into the lmo0880-deleted strain. The growth, adhesion, invasion, and intracellular proliferation were compared between the lmo0880-deleted strain, complementary strain, and the wild type. 【Results】 The deletion of lmo0880 did not significantly impact bacterial growth or adhesion. However, it led to notable decreases in cell invasion, proliferation, and colonization in the liver and spleen, ultimately diminishing the pathogenicity in mice. 【Conclusion】 The LPXTG-anchored protein Lmo0880 plays a crucial role in bacterial invasion, proliferation, and colonization in a host. These findings provide a solid foundation for deeply understanding the pathogen-host interaction during L. monocytogenes infection.