唾液宿主关联乳杆菌ATCC 11741益生特性

Probiotic characteristics of Ligilactobacillus salivarius ATCC 11741

摘要:

【目的】部分唾液宿主关联乳杆菌(Ligilactobacillus salivarius)菌株具备优良的益生特性和较好的应用潜力。以唾液宿主关联乳杆菌ATCC 11741菌株为研究对象，探究其生长特性、耐受性和黏附能力，以期为唾液宿主关联乳杆菌的有效利用提供理论基础。【方法】利用形态观察和16S rRNA基因测序确认唾液宿主关联乳杆菌ATCC 11741的准确性；通过监测生长曲线和产酸曲线来探究其生长特性；此外，还进行了一系列耐受性试验，包括耐酸、耐碱、耐胆盐、耐高渗和耐温测试，以评估其耐受能力；最后，借助自聚集和疏水性试验来间接测定其黏附性。【结果】唾液宿主关联乳杆菌ATCC 11741呈快速“S”型生长；产酸高峰期在2−7 h，14 h后趋于稳定，最终pH值稳定在4.3左右；该菌株在pH 4.0−11.0范围内生长良好，在pH 2.0的MRS培养基中培养4 h存活率为50.48%；在胆盐浓度为0.10%的MRS培养基中培养2 h之后的存活率可达到94.440 0%；在NaCl浓度为6%的MRS培养基中仍能生长；温度对其生长影响显著，30−42℃促进生长，而20℃和50℃则抑制其生长；转速为120 r/min和180 r/min对其生长无显著影响；该菌株在5 h时自聚集率为41.4%，疏水性(碳烃化合物黏着法)为44.5%。【结论】L. salivarius ATCC 11741是一株生长快，耐酸、耐碱、耐盐能力强，具有一定胆盐耐受性，温度适应性较广，具有较强黏附能力的乳酸菌。

Abstract:

[Objective]Certain strains ofLigilactobacillussalivariuspossess excellent probiotic properties and strong potential for application.This study characterized strain ATCC 11741 in terms of the growth characteristics,stress tolerance,and adhesion capacity,aiming to provide a theoretical basis for the utilization of L.salivarius. [Methods]L.salivarius ATCC 11741’s accuracy was verified through morphological observations and 16S rRNA gene sequencing;its growth characteristics were investigated by tracking growth and acid production curves;additionally,a range of tolerance tests,including those with acid,alkali,bile salts,hyperosmolarity and temperature,were conducted to evaluate its tolerance;lastly,self-aggregation and hydrophobicity experiments were used to indirectly determine its adhesion.[Results] The growth curve of L.salivarius ATCC 11741 was formed like a “S”.The strain reached its highest acid production between 2–7 h,stabilizing at pH 4.3 after 14 h.After being cultured in the MRS medium at pH 2.0 for 4 h,the strain had a survival rate of 50.48% and grew well in the pH 4.0–11.0 range.Following 2 h of incubation in the MRS medium with 0.10% bile salt,the strain’s survival rate was 94.440 0%.In the MRS medium with 6% NaCl,the strain continued to proliferate.The growth of strain ATCC 11741 was significantly affected by temperature,being promoted at 30–42℃ and inhibited at 20℃ and 50℃.At the 5 h mark,the strain’s hydrophobicity (measured by the hydrocarbon compound adhesion method) was 44.5% and its self-aggregation rate was 41.4%.[Conclusion] L.salivarius ATCC 11741 is a strain with fast growth,strong tolerance to acids,alkali,and salts,moderate tolerance to bile salt,a wide range of suitable temperatures,and strong adhesion capacity.