噪音环境对黄土边坡微生物群落的影响

Noise affects microbial communities on loess slopes

摘要:

【目的】 探讨不同噪音下黄土边坡土壤微生物在温度和时间影响下的变化。【方法】 通过测定土壤磷酸盐含量变化，确定噪音分贝在70、90、110 dB，温度在-5、15、35 ℃，噪音时间在2、4、6 h的条件下，土壤微生物群落的变化。随后对土壤微生物进行宏基因组学测序。【结果】 门水平上，放线菌门(Actinobacteria)、毛霉菌门(Mucoromycota)、热变形菌门(Thermoproteota)和黏球菌门(Myxococcota)组间存在显著差异(P<0.05)。属水平上，节杆菌属(Arthrobacter)、根孢囊霉属(Rhizophagus)、假节杆菌属(Pseudarthrobacter)、马杜拉放线菌属(Actinomadura)、考克氏菌属(Kocuria)、红色杆形菌属(Rubrobacter)和棒杆菌属(Corynebacterium)组间存在显著差异(P<0.05)；种水平上，Rhizophagus irregularis、Actinomadura sp. WMMB 499、热带红色杆形菌(Rubrobacter tropicus)、Arthrobacter sp. PGP41、Arthrobacter sp. 24S4 2和Arthrobacter crystallopoietes组间存在显著差异(P<0.05)。【结论】 不同噪音环境对门、属、种分类水平下的土壤微生物均具有显著影响。

关键词:黄土边坡;土壤微生物;噪音;温度;宏基因组

Abstract:

[Objective] To investigate the changes of microbial communities on loess slopes with different noise conditions under the influences of temperature and time. [Methods] Based on the changes in soil phosphate content, the noise was determined at 70, 90, and 110 dB. The microbial communities presented variations along the temperature gradients of -5, 15, and 35 °C and the noise duration of 2, 4, and 6 h. Subsequently, metagenomic sequencing was carried out for the soil microbial communities. [Results] At the phylum level, Actinobacteria, Mucoromycota, Thermoproteota, and Myxococcota showed differences in the relative abundance among groups (P<0.05). At the genus level, Arthrobacter, Rhizophagus, Pseudarthrobacter, Actinomadura, Kocuria, Rubrobacter, and Corynebacterium demonstrated different relative abundance among groups (P<0.05). At the species level, there were significant differences in the relative abundance of Rhizophagus irregularis, Actinomadura sp. WMMB 499, Rubrobacter tropicus, Arthrobacter sp. PGP41, Arthrobacter sp. 24S4 2, and Arthrobacter crystallopoietes among groups (P<0.05). [Conclusion] Different noise environments have significant effects on the relative abundance of soil microorganisms at the phylum, genus, and species levels.

Key words:loess slopes;soil microorganisms;noise;temperature;metagenome