Abstract:[Objective] To investigate the changes of microbial communities on loess slopes with different noise conditions under the influences of temperature and time. [Methods] Based on the changes in soil phosphate content, the noise was determined at 70, 90, and 110 dB. The microbial communities presented variations along the temperature gradients of -5, 15, and 35 °C and the noise duration of 2, 4, and 6 h. Subsequently, metagenomic sequencing was carried out for the soil microbial communities. [Results] At the phylum level, Actinobacteria, Mucoromycota, Thermoproteota, and Myxococcota showed differences in the relative abundance among groups (P<0.05). At the genus level, Arthrobacter, Rhizophagus, Pseudarthrobacter, Actinomadura, Kocuria, Rubrobacter, and Corynebacterium demonstrated different relative abundance among groups (P<0.05). At the species level, there were significant differences in the relative abundance of Rhizophagus irregularis, Actinomadura sp. WMMB 499, Rubrobacter tropicus, Arthrobacter sp. PGP41, Arthrobacter sp. 24S4 2, and Arthrobacter crystallopoietes among groups (P<0.05). [Conclusion] Different noise environments have significant effects on the relative abundance of soil microorganisms at the phylum, genus, and species levels.