1.福建农林大学 动物科学学院，福建 福州;2.福建省畜禽病原感染与免疫学重点实验室，福建 福州;3.闽台动物病原生物学福建省高校重点实验室，福建 福州
尤冬雪：实验操作、数据处理与分析、论文撰写与修改；潘宸：实验操作、数据收集和处理；陈玉章：论文讨论、协助实验操作；周欣霓：协助实验操作、数据处理；高铭：协助实验操作；陈梦颖：协助实验操作；王佳俊：协助实验操作；池晓娟：论文讨论、技术支持；王松：研究构思和设计、论文撰写和修改。
福建省自然科学基金(2020J06016)；福建农林大学科技创新专项基金(KFb22063XA)
- YOU Dongxue 1,2,3
YOU Dongxue
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China;Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China
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- CHEN Yuzhang 1,2
- ZHOU Xinni 1,2
- GAO Ming 1,3
- CHEN Mengying 1,3
- WANG Jiajun 1,3
- CHI Xiaojuan 1,2,3
CHI Xiaojuan
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China;Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China
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WANG Song
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China;Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China
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1.College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China;2.Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian, China;3.Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian, China
This work was supported by the Natural Science Foundation of Fujian Province (2020J06016) and the Science and Technology Innovation Project of Fujian Agriculture and Forestry University (KFb22063XA).
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目的 探究TGF-β1/Smad3在流感病毒WSN毒株感染过程中对类黏蛋白(orosomucoid, ORM)表达的调控作用。方法 利用流感病毒WSN毒株感染或TGF-β1处理A549细胞，然后使用Smad3抑制剂处理，通过RT-PCR检测ORM的表达情况；构建过表达STAT3的细胞系与敲低STAT3的细胞系，通过RT-PCR和Western blotting方法检测STAT3在流感病毒诱导ORM表达中的作用；使用TGF-β1处理A549细胞，通过Western blotting方法检测p-STAT3表达情况；使用TGF-β1或流感病毒处理A549细胞，然后使用Smad3抑制剂处理，检测p-STAT3表达情况；构建Smad3表达敲低细胞系，通过Western blotting方法检测STAT3磷酸化水平。结果 TGF-β1调控ORM1和ORM2的表达依赖于Smad3的活化；STAT3在流感病毒感染过程中参与了ORM1和ORM2的表达调控；TGF-β1能够诱导STAT3发生磷酸化；阻断Smad3的活化或敲低Smad3表达后，TGF-β1或WSN诱导的STAT3的磷酸化也被抑制。结论 流感病毒WSN毒株调控ORM1和ORM2的表达依赖于TGF-β1/Smad3介导的STAT3磷酸化。
Objective To investigate the regulatory effect of transforming growth factor-beta 1 (TGF-β1)/Smad3 on orosomucoid (ORM) expression during infection with influenza A/WSN/33 virus (hereinafter referred to as WSN).Methods A549 cells were either infected with WSN or treated with TGF-β1, followed by treatment with Smad3 inhibitors. The mRNA levels of ORMs were assessed by RT-PCR. Subsequently, cell lines with signal transducer and activator of transcription 3 (STAT3) overexpression and knockdown were established, and RT-PCR and Western blotting were employed to evaluate the impact of STAT3 on ORM expression induced by the influenza virus. Furthermore, A549 cells underwent treatment with TGF-β1, after which the phosphorylation status of STAT3 was analyzed via Western blotting. Finally, the phosphorylation level of STAT3 was detected after inhibiting the activity of Smad3 or knocking down the expression of Smad3 in A549 cells infected with WSN or treated with TGF-β1.Results TGF-β1 regulated the expression of ORM1 and ORM2 through the activation of Smad3, a key mediator in the TGF-β signaling pathway. Concurrently, STAT3 was implicated in modulating ORM1 and ORM2 expression during WSN infection. Additionally, TGF-β1 was shown to induce STAT3 phosphorylation. Notably, inhibiting Smad3 activation or knocking down Smad3 expression suppressed STAT3 phosphorylation.Conclusion The regulation of ORM1 and ORM2 expression by WSN relied on STAT3 phosphorylation mediated by TGF-β1/Smad3.
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尤冬雪,潘宸,陈玉章,周欣霓,高铭,陈梦颖,王佳俊,池晓娟,王松. 流感病毒
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- 收稿日期:2024-09-05
- 在线发布日期: 2025-02-18
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