靶向gat基因的shRNA重组微藻防控白纹伊蚊
作者:
作者单位:

1.佳木斯大学 生物与农业学院,黑龙江 佳木斯;2.中国热带农业科学院热带生物技术研究所,海南省海洋生物资源功能性成分研究与利用重点实验室, 海南 海口;3.海南医科大学 基础医学与生命科学学院,海南 海口;4.中国热带农业科学院湛江实验站,广东 湛江

作者简介:

唐欣欣:实验实施,结果分析,论文写作与修改;邓晓东:实验构思及设计,技术支持;黄小丹:实验实施;杨思琪:论文讨论及数据整理;薛春梅:论文讨论及修改;费小雯:实验构思及设计。

基金项目:

海南省重点研发计划(ZDYF2022SHFZ314);国家自然科学基金(82260669);海南省自然科学基金(322RC766);农业农村部财政专项(NFZX2024)


Recombinant expression of shRNA targeting gat in microalgae for controlling Aedes albopictus
Author:
Affiliation:

1.College of Biology and Agriculture, Jiamusi University, Jiamusi, Heilongjiang, China;2.Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bioresources in Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China;3.School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, Hainan, China;4.Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China

Fund Project:

This work was supported by the Key Research and Development Program of Hainan Province (ZDYF2022SHFZ314), the National Natural Science Foundation of China (82260669), the Natural Science Foundation of Hainan Province (322RC766), and the Financial Special Project of the Ministry of Agriculture and Rural Affairs (NFZX2024).

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    摘要:

    目的 探讨利用转基因微藻防控传播登革热等疾病的伊蚊的技术可行性。方法 以伊蚊神经递质转运体γ-氨基丁酸受体基因(gamma-aminobutyric acid receptor gene, gat)为靶点,构建shRNA微藻(Microalgae)表达载体,通过电击法将其转入莱茵衣藻(Chlamydomonas reinhardtii) CC124和小球藻(Chlorella vulgaris) HOC5中,获得重组微藻,并用其饲喂伊蚊幼虫和成蚊。结果 gat-shRNA转基因藻株可显著延缓伊蚊幼虫的发育、对伊蚊幼虫和成蚊表现出明显的致死效果,致死率分别为75.56%和58.67%。伊蚊幼虫体内目标基因gat的表达量显著降低。结论 在封闭水域中投放gat-shRNA转基因微藻以抑制水域附近的蚊子种群是可行的。该策略为使用生物方法控制伊蚊,以及阻断登革热、寨卡病毒病等严重传染病的传播提供了一种新的思路。

    Abstract:

    Objective To explore the technical feasibility of using transgenic microalgae to control Aedes that transmit diseases such as dengue fever.Methods Taking the neurotransmitter transporter gamma-aminobutyric acid receptor gene (gat) of Aedes as a target, we constructed a shRNA-expressing vector and then transferred the vector into Chlamydomonas reinhardtiii CC124 and Chlorella vulgaris HOC5 through electroporation. The recombinant microalgae were used to feed the larvae and adults of Aedes albopictus.Results The transgenic algae delayed the larval development and had obvious lethal effects on the larvae and adults of A. albopictus, causing the mortality rates of 75.56% and 58.67%, respectively. The expression level of gat in the larvae was significantly down-regulated.Conclusion Deploying gat-shRNA transgenic microalgae in enclosed water environments to suppress mosquito populations in the vicinity is technically feasible. This strategy provides a new perspective on using biological methods to control mosquitoes and block the transmission of severe infectious diseases such as dengue fever and Zika virus disease.

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唐欣欣,邓晓东,黄小丹,杨思琪,薛春梅,费小雯. 靶向gat基因的shRNA重组微藻防控白纹伊蚊[J]. 微生物学报, 2025, 65(5): 2240-2251

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  • 收稿日期:2024-12-04
  • 在线发布日期: 2025-04-30
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