单核增生李斯特氏菌LPXTG基序锚定蛋白Lmo0130的感染生物学作用

Infection ability of LPXTG motif-anchored protein Lmo0130 in Listeria monocytogenes

摘要:

目的 为了探究LPXTG基序锚定蛋白Lmo0130对单核增生李斯特氏菌EGD-e在感染致病中的影响，比较该菌EGD-e、lmo0130基因缺失株和回补株在生长、细胞和宿主感染等方面的差异。方法 构建单核增生李斯特氏菌lmo0130基因缺失株Δlmo0130和回补株CΔlmo0130，探究Lmo0130对单核增生李斯特氏菌的生长能力、细胞表面的细菌黏附与侵袭能力、细胞内的细菌增殖能力、细胞间的迁移能力，以及感染小鼠的存活能力和细菌定殖能力等方面的影响，从而阐明Lmo0130对单核增生李斯特氏菌在细胞和宿主感染中的作用。结果 LPXTG基序锚定蛋白Lmo0130有助于单核增生李斯特氏菌在细胞表面的黏附与侵袭、在细胞内的增殖、在感染小鼠组织中的定殖，并最终有助于在小鼠中的致病力，但对该菌的生长和胞间迁移能力无显著影响。结论 本研究阐明了Lmo0130在单核增生李斯特氏菌感染细胞和宿主中的作用。

Abstract:

Objective To investigate the role of Listeria monocytogenes LPXTG motif-anchored protein Lmo0130 in infection causing diseases, the bacterial growth, infection in cell and host among the L. monocytogenes wild-type, lmo0130-deleted and lmo0130-complementary strains were compared.Methods The lmo0130-deleted strain Δlmo0130 and lmo0130-complementary strain CΔlmo0130 were constructed to investigate the effects of Lmo0130 on the abilities of bacterial growth, cell surface adhesion and invasion, intracellular proliferation, intercellular migration, survival of infected mice, and bacterial load in mouse organs, ultimately demonstrated the role of L. monocytogenes Lmo0130 in cell and host infection.Results LPXTG motif-anchored protein Lmo0130 contributed to cell surface adhesion and invasion, intracellular proliferation, specific colonization in the liver and spleen, and pathogenicity in mice. However, it had no effect on bacterial growth or intercellular migration.Conclusion Lmo0130 contributes to cell and host infection of L. monocytogenes finally.