深海链霉菌NA13产环八肽surugamides的靶向分离及其植物促生活性发掘

Targeted isolation of cyclic octapeptide surugamides produced by deep-sea-derived Streptomyces sp. NA13 and exploration of their plant growth-promoting activities

摘要:

目的 靶向挖掘深海链霉菌(Streptomyces sp.) NA13中环八肽类化合物surugamides并筛选其生物活性。方法 结合基因组挖掘与LC-MS/MS分子网络分析，从深海链霉菌NA13中识别环肽类化合物，用天然产物化学手段分离与鉴定surugamides，并测定其抗菌活性及对水稻和玉米的促生长作用。结果 分离和鉴定4个环八肽化合物(surugamides A、B、D、E)，生物活性筛选结果表明，它们均能显著促进玉米和水稻幼苗根或茎的生长，其中surugamide A在0.1 μmol/L浓度下对玉米的促生根效果尤为突出。结论 本研究发现surugamides类化合物促进植物生长的新功能，为开发新型海洋微生物源植物生长调节剂提供了候选活性成分。

Abstract:

Objective To achieve the targeted isolation of the cyclooctapeptides, surugamides, from the deep-sea-derived Streptomyces sp. NA13 and explore their biological activities.Methods An approach integrating genome mining and LC-MS/MS molecular networking was employed to discover cyclopeptides from Streptomyces sp. NA13. Through systematic natural product isolation and characterization, these compounds were identified as surugamides. Their growth-promoting effects on Oryza sativa and Zea mays were assessed.Results Four cyclooctapeptides (surugamides A, B, D, and E) were isolated and identified. They had significant effects of promoting root growth in Z. mays and O. sativa seedlings. Notably, surugamide A at a concentration of 0.1 μmol/L demonstrated particularly outstanding growth-promoting effects on Z. mays roots.Conclusion This study uncovers the novel plant growth-promoting activity of surugamides, offering lead compounds for the development of innovative marine microbial-derived plant growth regulators.