产毒和非产毒霍乱弧菌在甘露醇 和Luria-Bertani培养液中的基因表达差异

Gene expression differences of toxigenic and nontoxigenic Vibrio cholerae strains in mannitol and Luria-Bertani broth

摘要:

摘要：【目的】分析霍乱弧菌产毒株和非产毒株在甘露醇发酵液和LB (Luria-Bertani) 培养液中生长的基因表达谱和代谢差异特征。【方法】提取甘露醇发酵液和LB培养液中霍乱弧菌甘露醇慢发酵株（产毒株）N16961和快发酵株（非产毒株）93097生长第一小时的总RNA，应用霍乱弧菌N16961基因组芯片分析各菌株在不同培养液中的表达差异基因。【结果】 筛选出产毒株N16961在甘露醇发酵液和LB中表达差异基因142个，非产毒株93097有418个，这些表达差异基因主要分属于6个不同的功能类群，主要是转运结合、能量代谢以及蛋白质合成代谢功能。【结论】甘露醇发酵液和LB中产毒株和非产毒株的许多功能基因的转录水平有显著差异，这些表达差异基因可能与霍乱弧菌在甘露醇发酵液中代谢产酸有关，这为进一步分析霍乱弧菌代谢甘露醇的机制、以及分析产毒株与非产毒株的甘露醇发酵快慢机制提供了基础。

Abstract:

Abstract: [Objective] To analyze gene expression differences of toxigenic and nontoxigenic strains of El Tor Vibrio cholerae growing separately in mannitol and LB (Luria-Bertani) fermentation medium. [Methods] Total RNA was extracted from the mannitol slow-fermenting strain N16961 (toxigenic) and the mannitol fast-fermenting strain 93097 (nontoxigenic) at 1 h of fermentation. The large scale gene expression profiles were detected and compared with high throughout microarray. [Results] By comparing the strains growing in different cultures, we found 142 differentially expressed genes in N16961 and 418 genes in 93097. Most of these genes were grouped into six functional classes. They were mainly related to transport and binding, energy metabolism, protein biosynthesis, and protein fate. [Conclusion] The expression levels of genes in N16961 and 93097 were affected by culture conditions, which can serve as basis for further studying the mechanism of metabolism of mannitol.