狂犬病病毒Evelyn- Rokitnicki-Abelseth疫苗株反向遗传系统的建立

Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain

摘要:

摘要：【目的】建立狂犬病毒的反向遗传系统，为研制不含狂犬病病毒致病性的新型安全高效的狂犬疫苗提供技术依据。【方法】本研究采用反向遗传学方法和分子克隆技术，建立了狂犬病病毒Evelyn- Rokitnicki-Abelseth (ERA)疫苗株的CMV/ T7、T7启动子病毒拯救系统，构建了表达N、P、L蛋白的辅助质粒。【结果】成功拯救出野生型病毒rERA-VC，在Vero细胞上的生长动力学特性与父母本ERA 相同，第三代可在Vero细胞上可获得很高的生长滴度。【结论】建立了狂犬病病毒的反向遗传系统，拯救出的野生型病毒生物学特性与父母本相同。

Abstract:

Abstract: [Objective] To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine. [Method] By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter. [Results] Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer. [Conclusion] The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.